Centro de Investigación y Asistencia Técnica a la Industria Agroalimentaria (CIATI AC), Neuquén, Argentina.
Int J Food Microbiol. 2011 Dec 2;151(2):229-34. doi: 10.1016/j.ijfoodmicro.2011.09.004. Epub 2011 Sep 10.
The purpose of this study was to determine the incidence of Alicyclobacillus spp. in fruit/vegetable juices (concentrated pulps and clarified and non-clarified juices) marketed in Argentina between 1996 and 2009. The presence of Alicyclobacillus was determined in a total of 8556 samples of fruit and vegetable juices (apple, pear, grape, peach, blend of juices, tangerine, pineapple, orange, mango, plum, guava, apricot, lemon, banana, kiwi, carrot, strawberry, grapefruit, and beetroot) collected in seven Argentinean provinces. Multiple factor analysis (MFA) was carried out on a data matrix that contained the percentage of positive samples, type of juice, raw material and production year. Except for kiwi and orange, Alicyclobacillus was found in juices from all the evaluated raw materials. The highest percentage of positive samples was found for beetroot, strawberry, banana, peach, mango, carrot and plum juices. The percentage of positive samples for these juices ranged from 100% to 24%. Furthermore, the application of multivariate techniques provided an insight on the relationship between the incidence of Alicyclobacillus and production variables. This approach enabled the identification of the most relevant variables that increased the percentage of positive samples among the juices, which could help in developing strategies to avoid the incidence of this bacterium. By means of hierarchical cluster analysis seven groups (clusters) of juices which showed different percentages of positive samples for Alicyclobacillus spp. were identified. This analysis showed that pineapple, peach, strawberry, mango and beetroot juices had higher rates of positivity for Alicyclobacillus than the rest of the evaluated juices. MFA analysis also showed that some clear relationships could be highlighted between the percentage of samples positive for Alicyclobacillus and five types of fruit juices (strawberry, beetroot, grapefruit, pineapple and mango). It was observed that a large proportion of juices produced in 2000, 2005 and 2008 were located in clusters with higher incidence of Alicyclobacillus spp., whereas a larger proportion of clarified concentrate juice and concentrate pulp samples showed higher probability of incidence of Alicyclobacillus in these products. Data presented in this study brings a contribution to the ecology of Alicyclobacillus in fruit/vegetable juices marketed in Argentina. This information would be useful to enhance the microbiological stability of fruit juices regarding the presence of Alicyclobacillus spp.
本研究的目的是确定 1996 年至 2009 年间在阿根廷销售的水果/蔬菜汁（浓缩浆和澄清及非澄清果汁）中脂环酸芽孢杆菌属的发生率。总共对 8556 份水果和蔬菜汁（苹果、梨、葡萄、桃、混合汁、橘子、菠萝、橙、芒果、李子、番石榴、杏、柠檬、香蕉、猕猴桃、胡萝卜、草莓、葡萄柚和甜菜根）样本进行了脂环酸芽孢杆菌检测，这些样本是在阿根廷的 7 个省采集的。对含有阳性样本百分比、果汁类型、原料和生产年份的数据矩阵进行了多因素分析（MFA）。除猕猴桃和橙子外，评估的所有原料果汁中均发现有脂环酸芽孢杆菌。甜菜根、草莓、香蕉、桃、芒果、胡萝卜和李子汁的阳性样本百分比最高。这些果汁的阳性样本百分比从 100%到 24%不等。此外，多元技术的应用提供了对脂环酸芽孢杆菌发生率与生产变量之间关系的深入了解。这种方法能够确定果汁中阳性样本百分比增加的最相关变量，这有助于制定避免这种细菌发生的策略。通过层次聚类分析，确定了 7 组（聚类）果汁，这些果汁的脂环酸芽孢杆菌属阳性样本百分比不同。分析表明，菠萝、桃、草莓、芒果和甜菜根汁的脂环酸芽孢杆菌阳性率高于评估的其他果汁。MFA 分析还表明，在某些水果汁（草莓、甜菜根、葡萄柚、菠萝和芒果）之间，可以发现脂环酸芽孢杆菌阳性样本百分比之间存在一些明显的关系。观察到 2000 年、2005 年和 2008 年生产的大部分果汁都位于脂环酸芽孢杆菌属阳性样本比例较高的聚类中，而澄清浓缩汁和浓缩浆样本的比例较高，则表明这些产品中脂环酸芽孢杆菌属的发生率较高。本研究提供了阿根廷市售水果/蔬菜汁中脂环酸芽孢杆菌生态学的相关信息。这些信息对于提高水果汁的微生物稳定性，防止脂环酸芽孢杆菌属的存在具有重要意义。
