Department of Biochemistry and Biophysics, Stockholm University, Stockholm, Sweden.
J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Nov 15;879(30):3393-400. doi: 10.1016/j.jchromb.2011.08.044. Epub 2011 Sep 10.
Aging is a complex multifactorial phenomenon, which is believed to result from the accumulation of cellular damage to biological macromolecules. Peroxisomes recently emerged as another important source of reactive oxygen species (ROS) production in addition to mitochondria. However, the role of these organelles in the process of aging is still not clear. The aim of this study was to characterize the changes in protein expression profiles of young (10 weeks old) versus old (18 months old) mouse liver peroxisome-enriched fractions. We have applied shotgun proteomic approach based on liquid chromatography and tandem mass spectrometry (LC-MS/MS) combined with iTRAQ (isobaric tags for relative and absolute quantitation) labeling that allows comparative quantitative multiplex analysis. Our analysis led to identification and quantification of 150 proteins, 8 out of which were differentially expressed between two age groups at a statistically significant level (p<0.05), with folds ranging from 1.2 to 4.1. These proteins involved in peroxisomal β-oxidation, detoxification of xenobiotics and production of ROS. Noteworthy, differences in liver proteome have been observed between as well as within different age groups. In conclusion, our subproteomic quantitative study suggests that mouse liver proteome is sufficiently maintained until certain age.
衰老是一种复杂的多因素现象，被认为是由于生物大分子的细胞损伤积累所致。过氧化物酶体除了线粒体之外，最近也成为活性氧（ROS）产生的另一个重要来源。然而，这些细胞器在衰老过程中的作用尚不清楚。本研究旨在描述年轻（10 周龄）和年老（18 月龄）小鼠肝过氧化物酶体富集部分的蛋白质表达谱变化。我们采用了基于液相色谱和串联质谱（LC-MS/MS）的 shotgun 蛋白质组学方法，并结合 iTRAQ（相对和绝对定量的同重同位素标记）标记，可进行比较定量多重分析。我们的分析鉴定和定量了 150 种蛋白质，其中 8 种在两个年龄组之间存在统计学上显著差异（p<0.05），倍数范围为 1.2 至 4.1。这些蛋白质参与过氧化物酶体β-氧化、外源物解毒和 ROS 产生。值得注意的是，在不同年龄组之间以及在同一年龄组内都观察到了肝脏蛋白质组的差异。总之，我们的亚蛋白质组定量研究表明，小鼠肝脏蛋白质组在一定年龄之前得到了充分的维持。
