多发性硬化症患者寡克隆带阴性时脑脊液和血液中的细胞因子图谱。

Cytokine mapping in cerebrospinal fluid and blood in multiple sclerosis patients without oligoclonal bands.

机构信息

Department of Neurology, Division of Neuroscience, Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Sweden.

出版信息

Mult Scler. 2012 May;18(5):669-73. doi: 10.1177/1352458511424903. Epub 2011 Sep 30.

DOI:10.1177/1352458511424903

PMID:21965416

Abstract

OBJECTIVE

Since there are clinical and genetic differences between MS patients with intrathecal oligoclonal bands (OCB+) in the cerebrospinal fluid (CSF) compared with those without (OCB-), the aim was to find out if OCB- patients showed a different pattern of cytokine immune activation compared with OCB+ patients.

METHODS

The study included 25 MS patients (10 OCB- and 15 OCB+) and 13 controls. A panel of cytokines was measured; IL-1β, IL-6, IL-8/CXCL8, IL-10, TNF and GM-CSF in serum, CSF and in supernatants from polyclonally stimulated blood mononuclear cells, where also levels of IL-12p40, IL-13, IL-15, IL-17 and IFN-γ were measured. The concentrations of soluble (s) VCAM-1 and sCD14 were measured in serum and CSF.

RESULTS

In general, there were no extensive differences in cytokine concentrations between the OCB- and OCB+ groups.

CONCLUSION

OCB- MS patients do not seem to constitute a separate entity concerning inflammatory parameters measured as cytokine concentrations in CSF and blood.

摘要

目的

由于脑脊髓液（CSF）中存在寡克隆带（OCB+）的 MS 患者与不存在寡克隆带（OCB-）的患者在临床和遗传方面存在差异，因此本研究旨在确定 OCB-患者是否与 OCB+患者表现出不同的细胞因子免疫激活模式。

方法

本研究纳入了 25 名 MS 患者（10 名 OCB-患者和 15 名 OCB+患者）和 13 名对照者。检测了一系列细胞因子，包括血清、CSF 和多克隆刺激的血单核细胞上清液中的 IL-1β、IL-6、IL-8/CXCL8、IL-10、TNF 和 GM-CSF，同时还检测了 IL-12p40、IL-13、IL-15、IL-17 和 IFN-γ的水平。血清和 CSF 中可溶性（s）VCAM-1 和 sCD14 的浓度也进行了测量。