Nano Science and Nano Technology program and Physics Department, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Biomed Microdevices. 2012 Feb;14(1):179-86. doi: 10.1007/s10544-011-9595-6.
In this paper, we report the construction of a polymerase chain reaction (PCR) device for fast amplification and detection of DNA. This device consists of an interchangeable PCR chamber, a temperature control component as well as an optical detection system. The DNA amplification happens on an interchangeable chip with the volumes as low as 1.25 μl, while the heating and cooling rate was as fast as 12.7°C/second ensuring that the total time needed of only 25 min to complete the 35 cycle PCR amplification. An optimized PCR with two-temperature approach for denaturing and annealing (Td and Ta) of DNA was also formulated with the PCR chip, with which the amplification of male-specific sex determining region Y (SRY) gene marker by utilizing raw saliva was successfully achieved and the genetic identification was in-situ detected right after PCR by the optical detection system.
本文报告了一种用于快速扩增和检测 DNA 的聚合酶链反应（PCR）装置的构建。该装置由可互换的 PCR 室、温度控制组件以及光学检测系统组成。DNA 扩增发生在一个体积低至 1.25 μl 的可互换芯片上，而加热和冷却速率高达 12.7°C/秒，确保总时间仅需 25 分钟即可完成 35 个循环的 PCR 扩增。还与 PCR 芯片一起制定了一种具有双温度法变性和退火（Td 和 Ta）的优化 PCR，利用该方法，成功地利用原始唾液扩增了雄性特异性性别决定区 Y（SRY）基因标记，并通过光学检测系统在 PCR 后直接进行了原位遗传鉴定。
