Department of Reproductive Medicine and Gynecologic Endocrinology, University Medical Centre Maribor, Maribor, Slovenia.
Fertil Steril. 2011 Dec;96(6):1473-1478.e2. doi: 10.1016/j.fertnstert.2011.09.015. Epub 2011 Oct 6.
To determine the influence of delayed compaction and fragmentation on the developmental capacity of morulas.
Prospective study.
University IVF center.
PATIENT(S): Intracytoplasmic sperm injection (ICSI) cycles with compact embryos on day 4 or day 5.
INTERVENTION(S): The embryos were divided into day 4 (n = 329) and day 5 (n = 256) morulas and graded I, II, or III, according to the percentage of fragmentation (<5%, 5%-20%, or >20%). The embryos were measured using Cronus3 software.
MAIN OUTCOME MEASUREMENT(S): Blastocyst development rate, blastocoel expansion rate, and optimal blastocyst rate. In an optimal blastocyst: surface area, trophectoderm cell number, inner cell mass (ICM) surface area, ICM volume and ICM shape.
RESULT(S): Day 4 morulas in classes I-III developed into optimal blastocysts in 57.4%, 50%, and 35.6% of the total, respectively, and day 5 morulas in classes I-III in 43.3%, 29.1%, and 13.6% of the total, respectively. A negative association was identified between the amount of morula fragmentation, the blastocyst ICM size, and the number of trophectoderm cells. A delay of 1 day in compaction was associated with a reduced ICM volume.
CONCLUSION(S): The measurement of compaction timing and cytoplasmic loss in morulas assists in predicting their ability to develop into optimal blastocysts.
确定延迟压缩和碎片化对桑葚胚发育能力的影响。
前瞻性研究。
大学试管婴儿中心。
卵胞浆内单精子注射（ICSI）周期中第 4 天或第 5 天有致密胚胎。
将胚胎分为第 4 天（n=329）和第 5 天（n=256）桑葚胚，并根据碎片百分比（<5%、5%-20%或>20%）分为 I、II 或 III 级。使用 Cronus3 软件对胚胎进行测量。
囊胚发育率、囊胚腔扩张率和优质囊胚率。优质囊胚：表面积、滋养外胚层细胞数、内细胞团（ICM）表面积、ICM 体积和 ICM 形状。
第 4 天 I-III 级桑葚胚分别有 57.4%、50%和 35.6%发育成优质囊胚，第 5 天 I-III 级桑葚胚分别有 43.3%、29.1%和 13.6%发育成优质囊胚。桑葚胚碎片数量、囊胚 ICM 大小和滋养外胚层细胞数量之间存在负相关。压缩时间延迟 1 天与 ICM 体积减少相关。
测量桑葚胚的压缩时间和细胞质丢失有助于预测其发育成优质囊胚的能力。
