Cueva R, Bordallo C, Suárez Rendueles P
Departamento de Biología Funcional, Universidad de Oviedo, Spain.
FEMS Microbiol Lett. 1990 May;57(1-2):153-7. doi: 10.1016/0378-1097(90)90430-x.
A yeast mutant lacking non-specific alpha-mannosidase activity was found as a background marker during our search for dap2 mutants (Suárez-Rendueles, P. and Wolf, D. H. (1987) J. Bacteriol. 169, 4041-4048). The mutant (DPS-15) is characterized in detail. The mutation called amd1 segregates 2:2 in meiotic tetrads, indicating a single chromosomal gene mutation which is recessive. Diploids heterozygous for amd1 show gene dosage. Thus, it appears that AMD1 might be the structural gene for alpha-mannosidase. Results obtained with this mutant show that alpha-mannosidase is not a vital component of the vegetative cell cycle. The differentiation process of sporulation is not disturbed in homozygous mutant diploids. Mannose turnover does not seem to be altered in mutant cells.
在我们寻找dap2突变体的过程中，发现了一个缺乏非特异性α-甘露糖苷酶活性的酵母突变体作为背景标记（苏亚雷斯-伦杜埃莱斯，P.和沃尔夫，D.H.（1987年）《细菌学杂志》169卷，4041 - 4048页）。对该突变体（DPS - 15）进行了详细表征。名为amd1的突变在减数分裂四分体中以2:2分离，表明这是一个隐性的单染色体基因突变。amd1杂合二倍体显示出基因剂量效应。因此，似乎AMD1可能是α-甘露糖苷酶的结构基因。用该突变体获得的结果表明，α-甘露糖苷酶不是营养细胞周期的关键组成部分。在纯合突变二倍体中，孢子形成的分化过程不受干扰。突变体细胞中的甘露糖周转率似乎没有改变。
