Health Sciences Post-graduate Program, Universidade Federal de Ciências da Saúde de Porto Alegre, Brazil.
APMIS. 2011 Nov;119(11):776-81. doi: 10.1111/j.1600-0463.2011.02807.x. Epub 2011 Sep 3.
Biofilm-forming staphylococci are known for being opportunistic and invasive pathogens that cause severe disease, mostly catheter-related infections. Early detection and pathogenic strains carrying highly transferable resistance cassettes epidemiology are essential for infection spread control. Hence, this study was designed to evaluate staphylococci biofilm formation and SCCmec typing. Biofilm production and SCCmec typing were evaluated using a semi-quantitative method based on microtiter plates and a multiplex PCR for types, I-V, respectively. Blood cultures and peripheral intravenous device (IVD) staphylococci were consecutively enrolled and allocated into two different groups (invasive and colonizing) based on clinical and microbiological criteria. Seventy-four invasive and 30 colonizing isolates from distinct patients were studied. Vancomycin was the most administrated antimicrobial agent among these patient's treatments. Biofilm formation was observed in 89% of invasive and 64% of colonizing isolates (p < 0.05). There was significant difference regarding SCCmec typing between colonizing and invasive isolates when harboring SCCmec types IV or V (p < 0.05), but no correlation between biofilm intensity and SCCmec types was verified. The SCCmec elements spread are still ongoing and for that reason, antimicrobial resistance evolution in invasive and colonizing biofilm-forming staphylococci is highly relevant.
生物膜形成的葡萄球菌已知是机会性和侵袭性病原体，会导致严重疾病，主要是导管相关感染。早期检测和携带高度可转移抗性盒的致病性菌株对于控制感染传播至关重要。因此，本研究旨在评估葡萄球菌生物膜形成和 SCCmec 分型。生物膜产生和 SCCmec 分型分别使用基于微量滴定板的半定量方法和用于类型 I-V 的多重 PCR 进行评估。连续纳入血培养和外周静脉装置 (IVD) 葡萄球菌，并根据临床和微生物学标准将其分配到两个不同的组（侵袭性和定植性）。研究了来自不同患者的 74 个侵袭性和 30 个定植性分离株。在这些患者的治疗中，万古霉素是使用最多的抗菌药物。在 89%的侵袭性和 64%的定植性分离株中观察到生物膜形成（p<0.05）。当携带 SCCmec 类型 IV 或 V 时，定植性和侵袭性分离株之间 SCCmec 分型存在显著差异（p<0.05），但生物膜强度与 SCCmec 类型之间没有相关性。SCCmec 元素的传播仍在继续，因此，侵袭性和定植性生物膜形成葡萄球菌中的抗菌药物耐药性演变非常重要。
