Farooqui Amber, Lei Yanchang, Wang Pusheng, Huang Jianyun, Lin Jie, Li Guishuang, Leon Alberto J, Zhao Zhen, Kelvin David J
Division of Immunology, International Institute of Infection and Immunity, Shantou University Medical College, 22 Xinling Road, Shantou, Guangdong 515041, China.
J Infect Dev Ctries. 2011 Oct 13;5(10):700-10. doi: 10.3855/jidc.2251.
South China has a proven role in the global epidemiology of previous influenza outbreaks due to its dual seasonal pattern. We present the virologic, genetic and clinical characterization of pandemic H1N1 influenza infection (pH1N1) in Shantou and Nanchang, cities in southern China, during the second wave of the 2009-2010 pandemic.
Nasopharyngeal swabs were collected from 165 individuals with influenza-like illness (ILI) who presented to the hospitals in Shantou and Nanchang. Laboratory diagnosis and characterization was performed by real-time PCR, virus isolation in embryonated chicken eggs, and sequencing.
pH1N1 activity was sustained in three different temporal patterns throughout the study period. The overall positivity rate of pH1N1 was 50% with major distribution among young adults between the ages of 13 and 30 years. High fever, cough, expectoration, chest pain, myalgia, nasal discharge and efficient viral replication were observed as major clinical markers whereas a substantial number of afebrile cases (17%) was also observed. Rate of hospitalization and disease severity (39%) and recovery (100%) were also high within the region. Furthermore, severe complications were likely to develop in young adults upon pH1N1 infection. Genetic characterization of the HA and NA genes of pH1N1 strains exhibited homogenous spread of pH1N1 strains with 99% identity with prototypic strains; however, minor unique mutations were also observed in the HA gene.
The study illustrates the detailed characteristics of 2009 influenza pandemic in southern parts of China that might help to strategize preparedness for future pandemics and subsequent influenza seasons.
由于华南地区具有双重季节性模式，在以往全球流感爆发的流行病学中已证实其发挥了作用。我们呈现了中国南方城市汕头和南昌在2009 - 2010年大流行第二波期间甲型H1N1流感大流行感染（pH1N1）的病毒学、遗传学和临床特征。
从汕头和南昌医院就诊的165例流感样疾病（ILI）患者中采集鼻咽拭子。通过实时PCR、鸡胚病毒分离和测序进行实验室诊断和特征分析。
在整个研究期间，pH1N1活动以三种不同的时间模式持续存在。pH1N1的总体阳性率为50%，主要分布在13至30岁的年轻人中。观察到高热、咳嗽、咳痰、胸痛、肌痛、流涕和高效病毒复制是主要临床指标，同时也观察到大量无发热病例（17%）。该地区的住院率、疾病严重程度（39%）和康复率（100%）也很高。此外，年轻人感染pH1N1后可能会出现严重并发症。pH1N1毒株的HA和NA基因的遗传特征显示pH1N1毒株具有同源传播，与原型毒株有99%的同一性；然而，在HA基因中也观察到了少量独特突变。
该研究阐明了中国南方2009年流感大流行的详细特征，这可能有助于制定未来大流行及后续流感季节的应对策略。
