Food Allergy Research and Resource Program, University of Nebraska, Lincoln, Nebraska, United States.
J Agric Food Chem. 2011 Dec 14;59(23):12309-16. doi: 10.1021/jf203277z. Epub 2011 Nov 3.
Parvalbumin is a pan-allergen in fish and frogs that triggers IgE-mediated reactions in fish-allergic individuals. Previous studies demonstrated that antibodies raised against fish and frog parvalbumins displayed varying specificity for different fish species, and thus, the applicability of these antibodies for potential use in immunoassays to detect fish residues were limited. We aimed to determine the specificity of 3 IgG antibodies for various fish species. Indirect enzyme-linked immunosorbent assay (ELISA) and IgG-immunoblotting were used to compare the reactivity of the polyclonal anticod parvalbumin antibody and the commercially available, monoclonal antifrog and monoclonal anticarp parvalbumin antibodies against raw muscle extracts of 29 fish species. All antibodies demonstrated varying specificities for different fish species. Of the 3 antibodies, the polyclonal anticod parvalbumin antibody is the most suitable for the detection of fish parvalbumins as it showed reactivity to the widest range of species, including herring, pilchard, carp, pike, cod, pollock, haddock, cusk, hake, bluegill, tilapia, bass, grouper, trout, catfish, and perch, although detection was still limited for several key fish species.
副肌球蛋白是鱼类和蛙类的泛过敏原，可引发鱼类过敏个体的 IgE 介导反应。先前的研究表明，针对鱼类和蛙类副肌球蛋白产生的抗体对不同鱼类物种表现出不同的特异性，因此，这些抗体在用于免疫分析以检测鱼类残留方面的适用性受到限制。我们旨在确定 3 种 IgG 抗体对各种鱼类的特异性。间接酶联免疫吸附测定（ELISA）和 IgG-免疫印迹用于比较多克隆抗副肌球蛋白抗体和市售的单克隆抗蛙和单克隆抗鲤鱼副肌球蛋白抗体对 29 种鱼类的原始肌肉提取物的反应性。所有抗体对不同鱼类物种的特异性不同。在这 3 种抗体中，多克隆抗副肌球蛋白抗体最适合检测鱼类副肌球蛋白，因为它对包括鲱鱼、沙丁鱼、鲤鱼、梭子鱼、鳕鱼、狭鳕、黑线鳕、长尾鳕鱼、无须鳕、牙鳕、蓝鳃太阳鱼、罗非鱼、鲈鱼、石斑鱼、鳜鱼、鳢鱼、鲶鱼和鲈鱼在内的最广泛的物种具有反应性，尽管对一些关键鱼类物种的检测仍然有限。
