Patel Y C, Amherdt M, Orci L
Endocrinology. 1979 Mar;104(3):676-9. doi: 10.1210/endo-104-3-676.
Monolayer cultures of neonatal rat pancreas have been characterized as an in vitro system for studying SRIF secretion. Marked 12- and 6-fold potentiation of SRIF release occurred with N-2-O-dibutyryl cAMP monosodium salt and theophylline, respectively. High glucose (300 mg/dl) stimulated SRIF release, whereas galactose was without effect. Exogenous insulin did not alter SRIF release, and the SRIF responses to theophylline and glucose were unaffected by the addition of antiinsulin serum to neutralize the insulin released by these agents. Arginine evoked a significant 2-fold increase in SRIF release. Exogenous glucagon produced slight but not significant stimulation of SRIF release. However, after exposure of the cultures to antiglucagon serum to diminish the concentration of glucagon in contact with the SRIF cells, exogenous glucagon produced a marked enhancement of SRIF secretion. These data suggest that glucose, arginine, glucagon, N-2-O-dibutyryl cAMP monosodium salt, and theophylline stimulate SRIF secretion, probably by direct effects on D cells or through mechanisms other than increased insulin secretion. Monolayer cultures of rat pancreas should provide a powerful in vitro system for studying pancreatic SRIF physiology.
新生大鼠胰腺的单层培养物已被表征为用于研究生长抑素（SRIF）分泌的体外系统。N - 2 - O - 二丁酰环磷腺苷钠盐和茶碱分别使SRIF释放显著增强了12倍和6倍。高糖（300mg/dl）刺激SRIF释放，而半乳糖则无作用。外源性胰岛素不改变SRIF释放，并且添加抗胰岛素血清以中和这些试剂释放的胰岛素后，SRIF对茶碱和葡萄糖的反应不受影响。精氨酸使SRIF释放显著增加2倍。外源性胰高血糖素对SRIF释放产生轻微但不显著的刺激。然而，在将培养物暴露于抗胰高血糖素血清以降低与SRIF细胞接触的胰高血糖素浓度后，外源性胰高血糖素使SRIF分泌显著增强。这些数据表明，葡萄糖、精氨酸、胰高血糖素、N - 2 - O - 二丁酰环磷腺苷钠盐和茶碱可能通过直接作用于D细胞或通过除增加胰岛素分泌以外的机制刺激SRIF分泌。大鼠胰腺的单层培养物应为研究胰腺SRIF生理学提供一个强大的体外系统。
