Wnt3a 参与诱导多能干细胞和胚胎干细胞造血分化的早期阶段。
Wnt3a is involved in the early stage of miPSC and mESC haemopoietic differentiation.
机构信息
Stem Cell and Regenerative Medicine Laboratory, Beijing Institute of Transfusion Medicine, Beijing, Peoples Republic of China.
出版信息
Cell Biol Int. 2012 Mar 1;36(3):267-71. doi: 10.1042/CBI20100766.
The Wnt/β-catenin signalling pathway is important in regulating not only self-renewal of haemopoietic progenitors and stem cells but also haemopoietic differentiation of ESCs (embryonic stem cells). However, it is still not clear how it affects haemopoietic differentiation. We have used a co-culture system for haemopoietic differentiation of mouse ESCs and iPSCs (induced pluripotent stem cells) in which the Wnt3a gene-modified OP9 cell line is used as stromal cells. The number of both Flk1+ and CD41+ cells generated from both co-cultured mouse ESCs and mouse iPSCs increased significantly, which suggest that Wnt3a is involved in the early stages of haemopoietic differentiation of mouse ESCs and mouse iPSCs in vitro.
Wnt/β-连环蛋白信号通路不仅在调节造血祖细胞和干细胞的自我更新方面很重要，而且在胚胎干细胞（ESCs）的造血分化方面也起着重要作用。然而，目前尚不清楚它如何影响造血分化。我们使用了一种共培养系统来进行小鼠 ESC 和 iPSC（诱导多能干细胞）的造血分化，其中 Wnt3a 基因修饰的 OP9 细胞系被用作基质细胞。来自共培养的小鼠 ESC 和小鼠 iPSC 的 Flk1+和 CD41+细胞的数量都显著增加，这表明 Wnt3a 参与了体外小鼠 ESC 和小鼠 iPSC 造血分化的早期阶段。
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