体内转染 BMP 2/VEGF(165)基因的小鼠骨髓基质细胞异位成骨。
Ectopic osteogenesis of mouse bone marrow stromal cells transfected with BMP 2/VEGF(165) genes in vivo.
机构信息
Department of Orthopaedics, People's Hospital of Guizhou Province, Guiyang, China.
出版信息
Orthop Surg. 2009 Nov;1(4):322-5. doi: 10.1111/j.1757-7861.2009.00045.x.
OBJECTIVE
To evaluate the osteogenic efficacy of mouse bone marrow stromal cells (mBMSC) transfected with bone morphogenetic protein 2 (BMP2) and vascular endothelial growth factor 165 (VEGF(165)) genes.
METHODS
pIRES-BMP2-VEGF(165) plasmid DNA was introduced into the mBMSC using a liposome-mediated method. The expression of BMP2 and VEGF(165) genes was assessed by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemical analysis. Transfected cells were injected into the thigh muscle pouches of four nude mice. The osteoinductivity activity of the transfected cells was evaluated by radiographic and histological analysis at 4 weeks after injection.
RESULTS
The mRNA and proteins of both BMP2 and VEGF(165) were successfully expressed in mBMSC as confirmed by RT-PCR and immunohistochemical analysis. Ectopically formed bone tissue was clearly observed at 4 weeks after cell injection in the thigh muscle pouches of the nude mice.
CONCLUSION
mBMSC transfected with BMP2 and VEGF(165) genes can induce ectopic osteogenesis.
目的
评估转染骨形态发生蛋白 2（BMP2）和血管内皮生长因子 165（VEGF(165)）基因的小鼠骨髓基质细胞（mBMSC）的成骨效果。
方法
采用脂质体介导的方法将 pIRES-BMP2-VEGF(165) 质粒 DNA 导入 mBMSC。通过逆转录-聚合酶链反应（RT-PCR）和免疫组织化学分析评估 BMP2 和 VEGF(165) 基因的表达。将转染的细胞注射到 4 只裸鼠的大腿肌肉囊中。注射后 4 周，通过影像学和组织学分析评估转染细胞的成骨活性。
结果
通过 RT-PCR 和免疫组织化学分析证实，BMP2 和 VEGF(165) 的 mRNA 和蛋白均在 mBMSC 中成功表达。在裸鼠大腿肌肉囊中细胞注射 4 周后，明显观察到异位形成的骨组织。
结论
转染 BMP2 和 VEGF(165) 基因的 mBMSC 可诱导异位成骨。
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