Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Osaka, Japan.
J Surg Oncol. 2012 Jun 15;105(8):780-5. doi: 10.1002/jso.22108. Epub 2011 Oct 19.
The aim of this study was to determine the expression of insulin-like growth factor-II messenger RNA (mRNA)-binding protein-3 (IMP-3) and its clinical significance in gastric cancers, as well the prognostic value of its expression in the peritoneal lavage fluid after surgery.
IMP-3 expression was examined by immunohistochemistry in 96 primary gastric tumors. IMP-3 mRNA expression in peritoneal lavage fluid obtained at laparotomy was determine by real-time quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).
Positive staining for IMP-3 was observed in 74% (71/96) of the tumors. IMP-3 expression in gastric tumors correlated significantly with worst overall survival (OS) and recurrence-free survival. Multivariate analyses identified pathological N stage and IMP-3 expression as significant independent prognostic factors for disease-free survival. Eight (28%) of 36 peritoneal lavage samples were cytologically negative but positive for IMP-3 mRNA expression by RT-PCR. The OS of patients with IMP-3-positive peritoneal lavage was significantly worse than of those with negative expression.
IMP-3 expression in primary gastric tumors was an independent poor prognostic factor. IMP-3 mRNA expression in peritoneal lavage fluid was a predictor of recurrence after surgery in gastric cancer and a marker of poor prognosis.
本研究旨在确定胰岛素样生长因子-II 信使 RNA（mRNA）结合蛋白-3（IMP-3）在胃癌中的表达及其临床意义，并研究其在手术后腹腔灌洗液中的表达的预后价值。
通过免疫组织化学法检测 96 例原发性胃癌肿瘤中 IMP-3 的表达。通过实时定量逆转录聚合酶链反应（RT-PCR）检测剖腹术中获得的腹腔灌洗液中 IMP-3 mRNA 的表达。
在 74%（71/96）的肿瘤中观察到 IMP-3 的阳性染色。胃癌肿瘤中 IMP-3 的表达与总生存（OS）和无复发生存率显著相关。多变量分析确定病理 N 期和 IMP-3 表达是无病生存的独立预后因素。36 个腹腔灌洗样本中有 8 个（28%）细胞学阴性，但 RT-PCR 检测 IMP-3 mRNA 表达阳性。IMP-3 阳性腹腔灌洗患者的 OS 明显差于阴性表达患者。
原发性胃癌肿瘤中 IMP-3 的表达是独立的不良预后因素。腹腔灌洗液中 IMP-3 mRNA 的表达是胃癌手术后复发的预测指标，也是预后不良的标志物。
