Laboratoire de Génétique, Biodiversité et Valorisation des Bio ressources (UR 03ES09), Institut Supérieur de Biotechnologie, Monastir, Tunisia.
Pharm Biol. 2011 Nov;49(11):1158-66. doi: 10.3109/13880209.2011.575791.
A large number of plants still need to be investigated through screening of amylases suitable for industry. In the present study, and for the first time, we describe the amylolytic activity of Saint Pedro Ficus carica L. (Moraceae) crude latex of Kahli and Bidhi varieties.
Effects of temperature, pH, metal ions, and inhibitors and compatibility with some commercial detergents were investigated for amylase activity.
Amylase activity was screened in crude latex using the DNS method and potato starch as a substrate. Analyses of amylolytic reaction products by thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) were performed.
Bidhi and Kahli amylases were active in optimal pH of 6.5 and 7 at 45°C, respectively, displaying a half life of 85 and 60 min, respectively, at 80°C, and they were very stable in a wide range of pH (4-12). Bidhi amylase activity increased to 260% by addition of 10(-3) mM Fe(2+) or 10(-2) mM Cu(2+), and was strongly inhibited by Mg(2+) and EDTA. In the presence of Ca(2+) and Mg(2+), Kahli amylase activity was dramatically enhanced by 220 and 260%, respectively. The compatibility of both amylases with certain commercial detergents was also shown to be good as enzymes retained up to 98% of their activities after 30 min of incubation at 80°C.
Analysis of amylolytic reaction products by TLC and HPLC suggested that Kahli amylase was an amyloglucosidase and Bidhi amylase was β-fructose, α(1-4) glucose. Bidhi amylase is a good choice for application in starch, food, detergents and medical industries.
大量的植物仍然需要通过筛选适合工业的淀粉酶进行研究。本研究首次描述了 Kahli 和 Bidhi 品种的圣佩德罗榕（桑科）粗乳胶的淀粉酶活性。
研究温度、pH 值、金属离子和抑制剂对酶活性的影响，以及与一些商业洗涤剂的兼容性。
采用 DNS 法和马铃薯淀粉作为底物，在粗乳胶中筛选淀粉酶活性。采用薄层色谱（TLC）和高效液相色谱（HPLC）分析淀粉酶反应产物。
Bidhi 和 Kahli 淀粉酶在 pH6.5 和 7 时分别在 45°C 下具有活性，半衰期分别为 80°C 下的 85 和 60 分钟，在 pH4-12 范围内非常稳定。Bidhi 淀粉酶活性在添加 10(-3)mMFe(2+)或 10(-2)mM Cu(2+)时增加到 260%，而在 Mg(2+)和 EDTA 存在下强烈抑制。在 Ca(2+)和 Mg(2+)存在下，Kahli 淀粉酶活性分别显著增强了 220%和 260%。两种淀粉酶与某些商业洗涤剂的兼容性也很好，因为酶在 80°C 孵育 30 分钟后仍保留 98%的活性。
TLC 和 HPLC 分析淀粉酶反应产物表明，Kahli 淀粉酶是一种淀粉酶，Bidhi 淀粉酶是β-果糖，α(1-4)葡萄糖。Bidhi 淀粉酶是淀粉、食品、洗涤剂和医疗行业应用的理想选择。
