体外评价矮茉莉叶甲醇提取物的抗菌和抗氧化活性。

In vitro evaluation of antimicrobial and antioxidant activities of methanolic extract of Jasminum humile leaves.

机构信息

MM College of Pharmacy, MM University, Mullana (Ambala)-133207, India.

出版信息

Asian Pac J Trop Med. 2011 Oct;4(10):804-7. doi: 10.1016/S1995-7645(11)60198-3.

DOI:10.1016/S1995-7645(11)60198-3

PMID:22014737

Abstract

OBJECTIVE

To evaluate in vitro antimicrobial and antioxidant activities of methanolic extract of Jasminum humile (J. humile) leaves extract.

METHOD

Methanolic extract of J. humile was evaluated for its antimicrobial activity by using agar well diffusion method & their possible antioxidant assay by two complementary test systems, namely DPPH and hydrogen peroxide scavenging activity. These various antioxidant activities were compared to standard antioxidants such as ascorbic acid for both the tests.

RESULTS

In the DPPH & hydrogen peroxide scavenging activity, the IC(50) value of methanol extract was 70.43 μg/mL & 60.79 μg/mL respectively. Further, the extract showed inhibitory activity for Gram-positive and negative bacteria at different concentrations. The maximum antibacterial activity of extract was exhibited against Staphylococcus aureus (S. aureus) at concentration 50 mg/mL when compared with ciprofloxacin

CONCLUSIONS

These results clearly indicate that J. humile is effective in scavenging free radicals and has the potential to be a powerful antioxidant. Thus, the results obtained in the present study indicate that J. humile leaves extract could be considered as a potential source of natural antioxidants and that could be used as an effective source against bacterial diseases.

摘要

目的

评估茉莉矮小（J. humile）叶提取物的甲醇提取物的体外抗菌和抗氧化活性。

方法

采用琼脂孔扩散法评估 J. humile 的甲醇提取物的抗菌活性，并使用两种互补的测试系统，即 DPPH 和过氧化氢清除活性，对其可能的抗氧化测定进行评估。将这些各种抗氧化活性与两种测试中的标准抗氧化剂（如抗坏血酸）进行比较。

结果

在 DPPH 和过氧化氢清除活性中，甲醇提取物的 IC（50）值分别为 70.43 μg/mL 和 60.79 μg/mL。此外，提取物在不同浓度下显示出对革兰氏阳性菌和革兰氏阴性菌的抑制活性。当与环丙沙星相比时，提取物在 50 mg/mL 的浓度下对金黄色葡萄球菌（金黄色葡萄球菌）表现出最大的抗菌活性。