South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong 510300, China.
Fish Shellfish Immunol. 2012 Jan;32(1):204-10. doi: 10.1016/j.fsi.2011.10.005. Epub 2011 Oct 14.
The chemokines regulate immune cell migration under inflammatory and physiological conditions. We investigated a CC chemokine gene (RcCC1) from cobia (Rachycentron canadum). The full-length RcCC1 cDNA is comprised 673 nucleotides and encodes a four-cysteine arrangement 99-amino-acid protein typical of known CC chemokines. The genomic DNA of RcCC1 consists of three exons and two introns. Phylogenetic analysis showed that RcCC1 was closest to the MIP group of CC chemokines. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed RcCC1 was constitutively expressed in all tissues examined, with relative strong expression in gill, blood, kidney, spleen, and head kidney. The RcCC1 transcripts in the head kidney, spleen, and liver were quickly up-regulated after stimulation with formalin-inactivated Vibrio carchariae (bacterial vaccine) or polyriboinosinic polyribocytidylic acid (poly I:C). These results indicate RcCC1 not only plays a role in homeostasis, but also may be involved in inflammatory responses to bacterial and viral infection.
趋化因子在炎症和生理条件下调节免疫细胞的迁移。我们研究了来自黄鳍金枪鱼（Rachycentron canadum）的 CC 趋化因子基因（RcCC1）。RcCC1 的全长 cDNA 由 673 个核苷酸组成，编码一个具有四个半胱氨酸排列的 99 个氨基酸蛋白，这是已知 CC 趋化因子的典型特征。RcCC1 的基因组 DNA由三个外显子和两个内含子组成。系统进化分析表明，RcCC1 与 MIP 组的 CC 趋化因子最为接近。定量实时 RT-PCR（qRT-PCR）分析显示，RcCC1 在所有检测的组织中均呈组成性表达，在鳃、血液、肾脏、脾脏和头肾中表达相对较强。用福尔马林灭活的创伤弧菌（细菌疫苗）或聚肌胞苷酸（poly I:C）刺激后头肾、脾脏和肝脏中的 RcCC1 转录物迅速上调。这些结果表明，RcCC1 不仅在体内平衡中发挥作用，而且可能参与对细菌和病毒感染的炎症反应。
