Kriz Petr, Slana Iva, Kralik Petr, Babak Vladimir, Skoric Misa, Fictum Petr, Docekal Jan, Pavlik Ivo
Department of Food and Feed Safety, Veterinary Research Institute, Hudcova 70, 621 00 Brno, Czech Republic.
Avian Dis. 2011 Sep;55(3):503-8. doi: 10.1637/9638-123010-Case.1.
An outbreak of Mycobacterium avium subsp. avium infection was diagnosed in one breed of domestic pigeons (Columba livia f. domestica) in the Czech Republic. Nodular granulomatous lesions were found in 42 (9.7%) pigeons of the 435 examined; histopathologic examination of livers with gross lesions of mycobacteriosis from 15 randomly selected pigeons revealed granulomatous inflammation typical for avian mycobacteriosis in all samples. Direct Ziehl-Neelsen (ZN) microscopy and conventional culture were performed for a total of 117 liver samples (42 pigeons with nodular lesions, 55 randomly selected pigeons without nodular lesions, and 20 randomly selected squabs). Acid-fast bacilli were observed in 19 (16.2%), and conventional culture yielded growth of M. a. avium in 40 (34.2%) liver samples. A triplex quantitative real-time PCR assay based on the IS901 detection system was performed successfully in 115 liver samples and revealed M. a. avium in 63 (54.8%) of them. Mycobacterium a. avium was also detected in two squabs. Eight domestic rabbits (Oryctolagus cuniculus f. domestica) living in the breeding facility were also examined. Pyogranulomatous lesions were only found in one adult male rabbit. At necropsy, both direct ZN microscopy and culture gave negative results for mycobacteria in all examined rabbit tissues. Mycobacterium a. avium was diagnosed in a liver sample of one juvenile rabbit using triplex qPCR, suggesting that M. a. avium infection can occur as early as juvenile animals.
在捷克共和国,一种家鸽(Columba livia f. domestica)品种中诊断出鸟分枝杆菌鸟亚种感染疫情。在检查的435只鸽子中,有42只(9.7%)发现有结节性肉芽肿病变;对随机选取的15只患有典型禽分枝杆菌病肝脏大体病变的鸽子进行组织病理学检查,结果显示所有样本均有典型的禽分枝杆菌病肉芽肿性炎症。对总共117份肝脏样本(42只患有结节性病变的鸽子、55只随机选取的无结节性病变的鸽子以及20只随机选取的雏鸽)进行了直接萋-尼(ZN)染色显微镜检查和传统培养。在19份样本(16.2%)中观察到抗酸杆菌,传统培养在40份肝脏样本(34.2%)中培养出鸟分枝杆菌鸟亚种。基于IS901检测系统的三重定量实时PCR检测在115份肝脏样本中成功进行,其中63份(54.8%)检测出鸟分枝杆菌鸟亚种。在两只雏鸽中也检测到鸟分枝杆菌鸟亚种。对生活在养殖设施中的8只家兔(Oryctolagus cuniculus f. domestica)也进行了检查。仅在一只成年雄性家兔中发现脓性肉芽肿病变。尸检时,所有检查的家兔组织的直接ZN染色显微镜检查和培养结果均显示分枝杆菌呈阴性。使用三重定量PCR在一只幼兔的肝脏样本中诊断出鸟分枝杆菌鸟亚种感染,这表明鸟分枝杆菌鸟亚种感染可能早在幼年动物中就会发生。