Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamada-oka, Suita, Osaka 565-0871, Japan.
J Biosci Bioeng. 2012 Jan;113(1):128-31. doi: 10.1016/j.jbiosc.2011.09.001. Epub 2011 Oct 20.
The procedure for fabricating a multilayered cell sheet has been developed by combining multiple sheets using a thermo-responsive surface and stamp system. Confocal laser scanning microscopy revealed that the fluidity of a multilayered sheet of skeletal myoblasts could be estimated as vertical diffusivity and changed upon addition of dermal fibroblasts.
通过使用温度响应表面和印花系统将多层片组合在一起,开发出了制造多层细胞片的方法。共聚焦激光扫描显微镜显示,骨骼肌成肌细胞的多层片的流动性可以作为垂直扩散系数来估计,并在加入真皮成纤维细胞后发生变化。