绿茶和姜多酚对人非小细胞肺癌细胞 h460 的生长抑制作用。
Growth inhibition of human non-small lung cancer cells h460 by green tea and ginger polyphenols.
机构信息
Department of Chemistry, Section of Biochemistry, Tanta University, Egypt.
出版信息
Anticancer Agents Med Chem. 2012 May;12(4):383-90. doi: 10.2174/187152012800228698.
UNLABELLED
Non small cell lung cancer is known to resist apoptotic stimuli of various antitumor agents and become progressively incurable. The present study was undertaken to evaluate the in vitro antineoplastic effect of polyphenols extracted from both green tea (GTPs) and ginger (GPs) on non-small cell lung cancer cells (NSCLC-NCI-H460).
METHODS
The direct antitumor effect of GTPs and GPs on H460 cells was assessed by investigating the proliferation rate, metabolic activity assay (MTT method) and the apoptotic effect (determined by an annexin V apoptosis assay). Also, the inhibition concentrations (IC50) of both extracts and the levels of P 53 and Bcl-2 proteins were determined.
RESULTS
GTPs and GPs have inhibited the proliferation of H460 cells in a dose-dependent manner. At the end of treatment period (96 h) the cell population has decreased to 16% and 26% when treated with 80μg GTPs or GPs, respectively, compared to the untreated cells. The IC50 values of both extracts were 32.9 and 55.5 g/ml, respectively. GTPs was more effective in reduction of cell metabolic activity (measured by MTT assay), where cell count decreased to 22% compared to 64% in cells treated with similar concentration (80μg) of GPs. Lower concentration (20μg) of cisplatin induced 15% reduction in cell metabolic activity. Moreover, 80μg of GTPs and GPs extracts induced apoptosis by 71% and 39% of the living cells, respectively. The apoptotic effect of both extracts, especially GTPs, seems to be mediated by both P 53 and Bcl-2.
CONCLUSION
The study reports the antiproliferative and apoptosis-mediated cytotoxic effects of green tea and ginger polyphenolic extracts on human H460 cell line, indicating their promising chemopreventive effect against lung cancer.
非小细胞肺癌已知对各种抗肿瘤药物的凋亡刺激具有抗性，并逐渐变得无法治愈。本研究旨在评估从绿茶（GTPs）和生姜（GPs）中提取的多酚对非小细胞肺癌细胞（NSCLC-NCI-H460）的体外抗肿瘤作用。
方法
通过研究增殖率、代谢活性测定（MTT 法）和凋亡作用（通过 Annexin V 凋亡测定确定）来评估 GTPs 和 GPs 对 H460 细胞的直接抗肿瘤作用。还测定了两种提取物的抑制浓度（IC50）和 P53 和 Bcl-2 蛋白的水平。
结果
GTPs 和 GPs 以剂量依赖性方式抑制 H460 细胞的增殖。在治疗期结束时（96 小时），与未经处理的细胞相比，用 80μg GTPs 或 GPs 处理的细胞群体分别减少到 16%和 26%。两种提取物的 IC50 值分别为 32.9 和 55.5μg/ml。GTPs 更有效地降低细胞代谢活性（通过 MTT 测定测量），其中与用类似浓度（80μg）的 GPs 处理的细胞相比，细胞计数减少到 22%。较低浓度（20μg）的顺铂诱导细胞代谢活性降低 15%。此外，80μg 的 GTPs 和 GPs 提取物分别诱导 71%和 39%的活细胞凋亡。两种提取物的凋亡作用，特别是 GTPs，似乎是由 P53 和 Bcl-2 介导的。
结论
本研究报告了绿茶和生姜多酚提取物对人 H460 细胞系的增殖抑制和凋亡介导的细胞毒性作用，表明它们对肺癌具有有希望的化学预防作用。
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