铁转运蛋白 OsNRAMP1 在水稻镉吸收和积累中的作用。
Role of the iron transporter OsNRAMP1 in cadmium uptake and accumulation in rice.
机构信息
Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
出版信息
Plant Signal Behav. 2011 Nov;6(11):1813-6. doi: 10.4161/psb.6.11.17587. Epub 2011 Nov 1.
Abstract
The heavy metal cadmium (Cd) is toxic to humans, and its accumulation in rice grains is a major agricultural problem. Rice has seven putative metal transporter NRAMP genes, but microarray analysis showed that only OsNRAMP1 is highly up-regulated by iron (Fe) deficiency. OsNRAMP1 localized to the plasma membrane and transported Cd as well as Fe. OsNRAMP1 expression was observed mainly in roots and was higher in the roots of a high-Cd-accumulating cultivar (Habataki) than in those of a low-Cd-accumulating cultivar (Sasanishiki). The amino acid sequence of OsNRAMP1 in the Sasanishiki and Habataki cultivars was found to be 100% identical. These results suggest that OsNRAMP1 participates in cellular Cd uptake and that the differences observed in Cd accumulation among cultivars are because of differences in OsNRAMP1 expression levels in roots.
摘要
重金属镉（Cd）对人体有毒，其在稻米中的积累是一个主要的农业问题。水稻有七个假定的金属转运体 NRAMP 基因，但微阵列分析表明，只有 OsNRAMP1 被铁（Fe）缺乏强烈上调。OsNRAMP1 定位于质膜，能够转运 Cd 和 Fe。OsNRAMP1 的表达主要在根中观察到，在高 Cd 积累品种（Habataki）的根中比在低 Cd 积累品种（Sasanishiki）的根中更高。在 Sasanishiki 和 Habataki 品种中发现 OsNRAMP1 的氨基酸序列完全相同。这些结果表明 OsNRAMP1 参与细胞 Cd 摄取，并且在品种间观察到的 Cd 积累差异是由于根中 OsNRAMP1 表达水平的差异。
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