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2008-2009 年从古巴出血性结膜炎患者中分离出柯萨奇病毒 A24 变种。

Isolation of Coxsackievirus A24 variant from patients with hemorrhagic conjunctivitis in Cuba, 2008-2009.

机构信息

Department of Virology, Pedro Kourí Tropical Medicine Institute (IPK), Autopista Novia del Mediodía km. 6 1/2, Marianao 13, Havana, Cuba.

出版信息

J Clin Virol. 2012 Jan;53(1):77-81. doi: 10.1016/j.jcv.2011.10.006. Epub 2011 Nov 9.

Abstract

BACKGROUND

An outbreak of acute hemorrhagic conjunctivitis occurred in Cuba in 2008 and 2009.

OBJECTIVE

To determinate the etiological agent associated with the Cuban outbreaks of acute hemorrhagic conjunctivitis during 2008 and 2009.

STUDY DESIGN

Conjunctival swabs and/or faecal samples from 382 patients with clinical diagnosis suggestive of acute hemorrhagic conjunctivitis were subject to viral culture in HEp-2 human laryngeal epidermoid carcinoma cells. Positive samples were identified by a specific Coxsackievirus A24 variant PCR and the 3C protease region of 16 isolates was sequenced for phylogenetic analysis.

RESULTS

Enterovirus cytopathic effect was observed in 138 cases (36%). A higher percent of CA24v was recovered from faecal samples, 19 out of 45 cases (42.2%), than from conjunctival swabs, 127 out of 355 samples (35.8%). All isolates were identified as Coxsackievirus A24 variant. Phylogenetic analysis revealed that 2008 and 2009 Cuban outbreaks were caused by the same virus strains and that isolates were closely related to those from Taiwan (2006-2007), China (2007-2008) and Singapore (2005) with a bootstrap value of 71%.

CONCLUSIONS

Outbreaks of acute hemorrhagic conjunctivitis occurred in Cuba in 2008 and 2009 were caused by Coxsackievirus A24 variant. The faecal-oral route is another mode of transmission of CA24v in the acute hemorrhagic conjunctivitis outbreaks. Phylogenetic analysis of Cuban CA24v strains involved in an acute hemorrhagic conjunctivitis outbreak in 2008 and 2009 confirms a new introduction of the CA24 variant into the Americas from South-east Asia.

摘要

背景

2008 年和 2009 年,古巴爆发了急性出血性结膜炎疫情。

目的

确定与 2008 年和 2009 年古巴急性出血性结膜炎疫情相关的病原体。

研究设计

对 382 例临床诊断为急性出血性结膜炎的患者的结膜拭子和/或粪便样本进行病毒培养,接种于人喉表皮样癌细胞株(HEp-2)。采用特异性柯萨奇病毒 A24 变体 PCR 鉴定阳性样本,并对 16 株分离株的 3C 蛋白酶区进行测序,进行系统进化分析。

结果

在 138 例(36%)患者的样本中观察到肠道病毒细胞病变效应。从粪便样本中分离出 19 株(42.2%)CA24v,高于从结膜拭子中分离出的 127 株(35.8%)。所有分离株均鉴定为柯萨奇病毒 A24 变体。系统进化分析显示,2008 年和 2009 年古巴疫情由同一病毒株引起,分离株与来自中国台湾(2006-2007 年)、中国大陆(2007-2008 年)和新加坡(2005 年)的病毒株密切相关,置信度为 71%。

结论

2008 年和 2009 年古巴爆发的急性出血性结膜炎疫情是由柯萨奇病毒 A24 变体引起的。粪-口途径是 CA24v 在急性出血性结膜炎疫情中传播的另一种方式。对 2008 年和 2009 年古巴急性出血性结膜炎疫情中涉及的 CA24 病毒株的系统进化分析证实,CA24 变体已从东南亚传入美洲。

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