Department of Molecular Biology and Biotechnology, Krebs Institute, University of Sheffield, Sheffield S10 2TN, UK.
Science. 2011 Nov 11;334(6057):821-4. doi: 10.1126/science.1211915.
The structure of BPSL1549, a protein of unknown function from Burkholderia pseudomallei, reveals a similarity to Escherichia coli cytotoxic necrotizing factor 1. We found that BPSL1549 acted as a potent cytotoxin against eukaryotic cells and was lethal when administered to mice. Expression levels of bpsl1549 correlate with conditions expected to promote or suppress pathogenicity. BPSL1549 promotes deamidation of glutamine-339 of the translation initiation factor eIF4A, abolishing its helicase activity and inhibiting translation. We propose to name BPSL1549 Burkholderia lethal factor 1.
BPSL1549 是一种来自伯克霍尔德氏菌的未知功能的蛋白质,其结构与大肠杆菌细胞毒性坏死因子 1 具有相似性。我们发现 BPSL1549 对真核细胞具有很强的细胞毒性,并且在给予小鼠时是致命的。bpsl1549 的表达水平与预期促进或抑制致病性的条件相关。BPSL1549 促进翻译起始因子 eIF4A 上的谷氨酰胺-339 的脱酰胺作用,从而使其丧失解旋酶活性并抑制翻译。我们建议将 BPSL1549 命名为伯克霍尔德氏菌致死因子 1。