Osada J, Sawaryn A, Dobryszycka W
Acta Biochim Pol. 1978;25(4):333-41.
Human haptoglobin (Hp) of the 1-1 type incorporated one spin or fluorescence marker per molecule; the markers were found in the beta chain. Formation of the complex between spin-labelled Hp and haemoglobin or antibody caused conformational changes in the Hp molecular, evidenced by increased participation in the electron paramagnetic resonance spectrum of the component bound with the slowly rotating marker. From fluorescence-labelled Hp, the beta chain was isolated and cleaved by CNBr; only in one of the obtained peptides, one out of 4 histidine residues was modified with the marker.
1-1型人触珠蛋白（Hp）每个分子结合一个自旋或荧光标记；这些标记位于β链中。自旋标记的Hp与血红蛋白或抗体之间形成复合物会导致Hp分子发生构象变化，这可通过与缓慢旋转标记结合的组分在电子顺磁共振谱中的参与度增加来证明。从荧光标记的Hp中分离出β链并用溴化氰进行切割；在所获得的肽中，只有一个肽段中的4个组氨酸残基中有一个被标记修饰。
