从鸡蛋果中提取的白花丹素对胶原诱导性关节炎中 T 细胞增殖和细胞因子反应的调节作用。

Modulation of T cell proliferation and cytokine response by Plumbagin, extracted from Plumbago zeylanica in collagen induced arthritis.

机构信息

Department of Biochemistry, Andhra University, Visakhapatnam, Andhra Pradesh, India.

出版信息

BMC Complement Altern Med. 2011 Nov 16;11:114. doi: 10.1186/1472-6882-11-114.

BACKGROUND

The extracts of Plumbago zeylanica have been used in China and other Asian countries as folk medicine for the treatment of cancer, rheumatoid arthritis and dysmenorrhoea. Effect of Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) purified from Plumbago zeylanica on Con A induced T cell proliferation was studied in spleen cells from collagen induced arthritic DBA/1 mice.

METHODS

The DBA/1 mice (five per each group) were immunized with 0.1 mL of collagen (emulsified in CFA) by intradermal injection at the base of the tail. On day 20, mice were given a booster dose of collagen (emulsified in IFA) through the same route. Plumbagin was given at different concentrations (3.3, 6.6, 13.3 mg/kg body weight) intraperitoneally. Control mice received olive oil alone. The Con A induced T cell proliferative responses of arthritic and Plumbagin treated mice were studied by cell culture experiments using tritiated Thymidine. In addition the cytokine levels were estimated from the in vitro spleen culture supernatants of arthritic mice primed with different concentrations of Plumbagin by ELISA.

RESULTS

Plumbagin enhanced the decreased Con A induced T cell proliferation and Interleukin-2 production in arthritic mice. Moreover elevated levels of IFN- γ were found to be decreased in Plumbagin treated spleen cell culture supernatants. Subclasses of IgG were found to be decreased by Plumbagin treatment, IgG2a reduction seems to be more prominent.

CONCLUSION

The results obtained in the current study indicate that Plumbagin is very effective in the mechanism based treatment of Rheumatoid arthritis.

背景

在中国和其他亚洲国家，白花丹已被用作民间药物，用于治疗癌症、类风湿性关节炎和痛经。本研究从白花丹中提取的有效成分白花丹醌（5-羟基-2-甲基-1,4-萘醌），研究其对胶原诱导关节炎 DBA/1 小鼠脾细胞中 ConA 诱导的 T 细胞增殖的影响。

方法

将 DBA/1 小鼠（每组 5 只）通过尾根部皮内注射 0.1ml 胶原（在 CFA 中乳化）进行免疫。第 20 天，通过相同途径给予胶原（在 IFA 中乳化）的加强剂量。白花丹以不同浓度（3.3、6.6、13.3mg/kg 体重）腹腔内给药。对照组小鼠给予橄榄油。通过氚标记胸腺嘧啶细胞培养实验研究关节炎和白花丹治疗小鼠的 ConA 诱导的 T 细胞增殖反应。此外，通过 ELISA 从用不同浓度白花丹预刺激的关节炎小鼠的体外脾培养上清液中估计细胞因子水平。