Department of Clinical Neurosciences, University of Calgary, 3330 Hospital Dr NW, Calgary, Alberta T2N 4N1, Canada; Polish Academy of Sciences, Institute of Nuclear Physics, Krakow, 152 Radzikowskiego, Krakow, Malopolska 31-342, Poland.
Institute for Biodiagnostics, National Research Council of Canada, 435 Ellice Avenue, Winnipeg, Manitoba R3B 1Y6, Canada.
J Neurosci Methods. 2012 Feb 15;204(1):118-123. doi: 10.1016/j.jneumeth.2011.10.030. Epub 2011 Nov 10.
Both 0.2 T and 9.4 T MRI systems were used to image a mouse model of glioma. RF coils were designed for both fields. A spin-echo, multi-echo pulse sequence was used to determine T(2) relaxation times of both brain and tumor tissues. Contrast-to-noise ratio was calculated based on the selected echo time. The results showed that 0.2 T is suitable for mouse model imaging, however total scan time must be long to achieve high enough SNR. T(2) relaxation times of the tumor and brain tissues can be measured at 0.2 T and are 2.1 and 1.8 times respectively longer at 0.2 T than at 9.4 T. Contrast to noise ratio for tumor and brain was better at high field than at the low field. We concluded that 0.2 T may be used to study mouse model of glioma using spin echo pulse sequence, yet the total scan time is long (about 40 min), resolution is lower (∼250 μm × 250 μm) and slice thickness (3mm) must be large enough to obtain sufficient SNR.
分别使用 0.2T 和 9.4T MRI 系统对胶质瘤小鼠模型进行成像。为两个场设计了射频线圈。使用自旋回波，多回波脉冲序列来确定脑和肿瘤组织的 T(2)弛豫时间。根据所选回波时间计算对比噪声比。结果表明，0.2T 适用于小鼠模型成像，但是要达到足够高的 SNR，总扫描时间必须延长。在 0.2T 下测量肿瘤和脑组织的 T(2)弛豫时间，分别比在 9.4T 下长 2.1 和 1.8 倍。高场的肿瘤和脑的对比噪声比优于低场。我们得出结论，0.2T 可用于使用自旋回波脉冲序列研究胶质瘤小鼠模型，但总扫描时间较长（约 40 分钟），分辨率较低（约 250μm×250μm），且切片厚度（3mm）必须足够大，以获得足够的 SNR。
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