Department of Physical Chemistry, Israel Institute for Biological Research, Ness Ziona, Israel.
Adv Exp Med Biol. 2012;733:47-52. doi: 10.1007/978-94-007-2555-3_5.
A new form of high surface bioelectrode based on electrospun gold microfiber with -immobilized glucose oxidase was developed. The gold fibers were prepared by electroless deposition of gold nanoparticles on a poly(acrylonitrile)-HAuCl(4) electrospun fiber. The material was characterized using electron microscopy, XRD and BET, as well as cyclic voltammetry and biochemical assay of the immobilized enzyme. The surface area of the gold microfibers was 2.5 m(2)/g. Glucose oxidase was covalently crosslinked to the gold surface using cystamine monolayer and glutardialdehyde, and portrayed characteristic catalytic currents for oxidizing glucose using a ferrocene methanol mediator. Limit of detection of glucose is 0.1 mM. The K(m) of the immobilized enzyme is 10 mM, in accordance with other reports of immobilized glucose oxidase. The microfiber electrode was reproducible and showed correlation between fiber weight, cathodic current and enzymatic loading.
一种新型高比表面生物电极，基于静电纺丝金微纤维与 -固定化葡萄糖氧化酶。金纤维是通过化学镀方法在聚（丙烯腈）-HAuCl(4)静电纺纤维上沉积金纳米粒子制备的。利用电子显微镜、XRD 和 BET 以及循环伏安法和固定化酶的生化分析对材料进行了表征。金微纤维的比表面积为 2.5 m(2)/g。使用半胱胺单层和戊二醛将葡萄糖氧化酶共价交联到金表面，并使用二茂铁甲醇介体描绘出氧化葡萄糖的特征催化电流。葡萄糖的检测限为 0.1 mM。固定化酶的 K(m)为 10 mM，与其他固定化葡萄糖氧化酶的报道一致。微纤维电极具有重现性，并显示出纤维重量、阴极电流和酶负载之间的相关性。
