Nestel P J, Reardon M, Billington T
Biochim Biophys Acta. 1979 May 25;573(2):403-7. doi: 10.1016/0005-2760(79)90073-0.
The fate of cholesteryl esters in high density lipoprotein (HDL) was studied to determine whether the transfer of esterified cholesterol from HDL to other plasma lipoproteins occurred to a significant extent in man. HDL cholesteryl ester, labelled in vitro with [3H] cholesterol, was injected into human subjects. Labelling of cholesteryl esters in very low density (VLDL) occurred rapidly and by 3 h, the esterified cholesterol in VLDL reached peak specific radioactivity. The removal rate of cholesteryl esters from HDL appeared to be exponential and of the order of 0.2/h; calculation of the apparent flux was about 150 mg/h which approximates reported values for total cholesterol esterification in human plasma in vivo. The rapid rate of labelling of VLDL from HDL suggests that the transfer of HDL cholesteryl esters to VLDL may represent a significant pathway for the disposal of HDL cholesterol.
研究了高密度脂蛋白（HDL）中胆固醇酯的命运，以确定酯化胆固醇从HDL转移至其他血浆脂蛋白的现象在人体内是否显著发生。将经体外[3H]胆固醇标记的HDL胆固醇酯注入人体受试者体内。极低密度脂蛋白（VLDL）中胆固醇酯的标记迅速发生，至3小时时，VLDL中的酯化胆固醇达到放射性比活峰值。HDL中胆固醇酯的清除率似乎呈指数形式，约为0.2/h；表观通量的计算值约为每小时150mg，这与体内人体血浆中总胆固醇酯化的报道值相近。HDL中胆固醇酯快速标记VLDL表明，HDL胆固醇酯向VLDL的转移可能是HDL胆固醇清除的一条重要途径。
