Aydin Fatma, Levent Yildiz, Nilgun Senturk, Pancar Yuksel Esra, Yasar Turanli Ahmet
Ondokuz Mayis University, School of Medicine, Departments of Dermatology and Pathology, Samsun, Turkey.
Indian J Dermatol. 2011 Sep-Oct;56(5):501-4. doi: 10.4103/0019-5154.87130.
The underlying molecular basis of mycosis fungoides (MF) has not yet been clarified. However, defects in apoptosis may contribute to its pathogenesis.
We investigated the expression of Bax, Fas, and p53 in early-stage MF patients and any alterations in expression following photochemotherapy.
Bax, Fas, and p53 expressions were studied by immunohistochemistry in both keratinocytes and lymphocytes on paraffin-embedded skin specimens from 27 early-stage MF patients.
Bax, Fas, and p53 staining was shown in the lymphocytes in 0/27, 26/27, and 11/27 patients at the time of diagnosis, whereas these ratios were 0/27, 9/27, and 0/27, respectively, after psoralen plus ultraviolet A (PUVA) treatment. The decrease in p53 and Fas expression in the lymphocytes was found statistically significant. Bax, Fas, and p53 staining in the keratinocytes was shown in 5/27, 27/27, and 25/27 patients at the time of diagnosis, whereas these ratios were 0/27, 22/27, and 4/27, respectively, after PUVA treatment. The decrease in p53, Fas, and Bax expression in the keratinocytes was found statistically significant.
Although Bax seems unrelated with early-stage MF, Fas and p53 expression in the lymphocytes may contribute to the understanding of the pathogenesis of this disease.
蕈样肉芽肿(MF)的潜在分子基础尚未阐明。然而,细胞凋亡缺陷可能在其发病机制中起作用。
我们研究了早期MF患者中Bax、Fas和p53的表达以及光化学疗法后表达的任何变化。
通过免疫组织化学研究了27例早期MF患者石蜡包埋皮肤标本中角质形成细胞和淋巴细胞中的Bax、Fas和p53表达。
诊断时,27例患者中淋巴细胞Bax、Fas和p53染色阳性的分别为0/27、26/27和11/27,而补骨脂素加紫外线A(PUVA)治疗后,这些比例分别为0/27、9/27和0/27。淋巴细胞中p53和Fas表达的降低具有统计学意义。诊断时,27例患者中角质形成细胞Bax、Fas和p53染色阳性的分别为5/27、27/27和25/27,而PUVA治疗后,这些比例分别为0/2。角质形成细胞中p53、Fas和Bax表达的降低具有统计学意义。
虽然Bax似乎与早期MF无关,但淋巴细胞中Fas和p53的表达可能有助于理解该疾病的发病机制。 7、22/27和4/27。