免疫亲和柱在线柱后光化学衍生化-HPLC-fLD法同时测定甘草中黄曲霉毒素B1、B2、G1、G2及赭曲霉毒素A
[Simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-fLD after immunoaffinity column with online post-column photochemical derivatization].
作者信息
Wei Riwei, Yang Xiaoli, Qiu Feng, Yang Meihua, Qin Jieping
机构信息
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2011 Sep;36(17):2342-6.
OBJECTIVE
To develop a method for the simultaneous determination of aflatoxin B1, B2, G1, G2 and ochratoxin A in Glycyrrhiza uralensis by HPLC-FLD after immunoaffinity column with online post-column photochemical derivatization.
METHOD
Sample was extracted with MeOH: H2O (80:20) and cleaned up by immunoaffinity column. The toxins were separated by reversed-phase HPLC and the mobile phase was consisted of methanol and 0.5% acetic acid solution with gradient elution. The determination was carried out by fluorescence detector after photochemical derivatization.
RESULT
The detection limits of aflatoxin G2, G1, B2, B1 and ochratoxin A were 0.02, 0.06, 0.015, 0.03 and 0.25 microg x kg(-1), respectively. The recoveries of analytes were from 76.0% to 103% and the relative standard deviations (RSDs) were below 13%.
CONCLUSION
The method is a simple, accurate and can be used to determine the contents of aflatoxin B1, B2, G1, G2 and ochratoxin A in G. uralensis simultaneously.
目的
建立一种采用免疫亲和柱净化并在线柱后光化学衍生化，通过高效液相色谱 - 荧光检测器（HPLC - FLD）同时测定甘草中黄曲霉毒素B1、B2、G1、G2和赭曲霉毒素A的方法。
方法
样品用甲醇∶水（80∶20）提取，经免疫亲和柱净化。毒素采用反相高效液相色谱分离，流动相由甲醇和0.5%乙酸溶液组成，梯度洗脱。光化学衍生化后用荧光检测器进行测定。
结果
黄曲霉毒素G2、G1、B2、B1和赭曲霉毒素A的检出限分别为0.02、0.06、0.015、0.03和0.25 μg·kg⁻¹。分析物的回收率为76.0%至103%，相对标准偏差（RSDs）低于13%。
结论
该方法简便、准确，可用于同时测定甘草中黄曲霉毒素B1、B2、G1、G2和赭曲霉毒素A的含量。
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