烧伤治疗的新概念：控释型粒细胞-单核细胞集落刺激因子壳聚糖凝胶系统

A new concept in treatment of burn injury: controlled slow-release granulocyte-monocyte colony-stimulating factor chitosan gel system.

作者信息

Simsek Serife, Canter Halil Ibrahim, Konas Ersoy, Korkusuz Petek, Demir Devrim, Oner Filiz, Unsal Ibrahim, Mavili Mehmet Emin

机构信息

Acibadem Health Group, Department of General Surgery, Atasehir Hospital, Istanbul, Turkey.

出版信息

Ann Plast Surg. 2011 Dec;67(6):583-8. doi: 10.1097/SAP.0b013e31822af865.

DOI:10.1097/SAP.0b013e31822af865

PMID:22123539

Abstract

OBJECTIVE

The aim of the study is to investigate the effectiveness of the controlled slow-release granulocyte-monocyte colony-stimulating factor (GM-CSF) system in burn wound healing.

MATERIAL AND METHODS

In vivo effect of controlled slow-release GM-CSF from chitosan gel on burn wound healing was evaluated on 18 Wistar-Albino rats, weighing between 250 and 300 g. They were randomly divided into 3 groups; (1) burned only group (n = 6), (2) burned + chitosan group (n = 6), (3) burned + chitosan + GM-CSF group (n = 6). Wound area was measured macroscopically. Hematoxylin and eosin and Masson's trichrome stained sections were evaluated for wound healing and tissue response to the polymer.

RESULTS

The best healing process was observed with the controlled slow-release GM-CSF-applied group (group 3) in which the wound area was significantly narrowed.

CONCLUSION

The study demonstrated the positive contribution of the single-dose controlled slow-release GM-CSF from chitosan gel on burn wound healing.

摘要

目的

本研究旨在探讨可控缓释粒细胞-单核细胞集落刺激因子（GM-CSF）系统对烧伤创面愈合的有效性。

材料与方法

对18只体重在250至300克之间的Wistar-白化大鼠评估壳聚糖凝胶中可控缓释GM-CSF对烧伤创面愈合的体内作用。它们被随机分为3组；（1）仅烧伤组（n = 6），（2）烧伤+壳聚糖组（n = 6），（3）烧伤+壳聚糖+GM-CSF组（n = 6）。宏观测量创面面积。对苏木精-伊红染色和Masson三色染色切片进行创面愈合及组织对聚合物反应的评估。