National Cancer Center, Goyang, Republic of Korea.
Int J Gynecol Cancer. 2011 Dec;21(9):1566-72. doi: 10.1097/IGC.0b013e318226c5f5.
This study was undertaken to evaluate protein and lipid profiles of ovarian cancer tissue samples. Twenty-three frozen ovarian cancer samples and 6 adjacent normal samples were analyzed using histology-directed, matrix-assisted laser desorption/ionization mass spectrometry. Sinapinic acid and 2, 5-dihydroxybenzoic acid/α-cyano-4-hydroxycinnamic acid were manually deposited on areas of each tissue section enriched in epithelial cells to identify protein and lipid profiles respectively, and mass spectra were acquired using a matrix-assisted laser desorption/ionization-time of flight instrument. Protein and lipid profiles classify 11 cancer and 3 adjacent normal samples in 100 random test sets with 92.9% median accuracy. Phosphatidylcholines {32:3} [M + Na] (m/z = 750.66), {34:1} [M + K] (m/z = 798.60), and {36:2} [M + K] (m/z = 824.56) were found to be increased in ovarian cancer. Interestingly, breast cancer-associated changes in lipid and protein profiles were also found in ovarian cancer. Thus, protein and lipid profiles accurately distinguish ovarian cancer from adjacent normal tissue samples. Common cancer-associated alterations in lipid and protein profiles were identified between ovarian and breast cancers.
本研究旨在评估卵巢癌组织样本的蛋白质和脂质谱。使用组织学导向的基质辅助激光解吸/电离质谱法分析了 23 个冷冻卵巢癌样本和 6 个相邻正常样本。分别使用 2,5-二羟基苯甲酸/α-氰基-4-羟基肉桂酸和芥子酸手动沉积在富含上皮细胞的每个组织切片区域，以分别鉴定蛋白质和脂质谱，并使用基质辅助激光解吸/电离-飞行时间仪器获取质谱。蛋白质和脂质谱以 92.9%的中位数准确率将 100 个随机测试集中的 11 个癌症和 3 个相邻正常样本分类。发现卵巢癌中磷脂酰胆碱 {32:3} [M + Na]+（m/z = 750.66），{34:1} [M + K]+（m/z = 798.60）和{36:2} [M + K]+（m/z = 824.56）增加。有趣的是，在卵巢癌中也发现了与乳腺癌相关的脂质和蛋白质谱变化。因此，蛋白质和脂质谱可准确地区分卵巢癌与相邻正常组织样本。在卵巢癌和乳腺癌之间确定了脂质和蛋白质谱中常见的癌症相关改变。
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