利用扫描力显微镜和扫描电子显微镜研究纳米技术中的番茄丛矮病毒。

Tomato bushy stunt viruses (TBSV) in nanotechnology investigated by scanning force and scanning electron microscopy.

机构信息

Department of Physics and Research Center OPTIMAS, University of Kaiserslautern, Kaiserslautern, Germany.

出版信息

Colloids Surf B Biointerfaces. 2012 Mar 1;91:154-61. doi: 10.1016/j.colsurfb.2011.10.048. Epub 2011 Nov 3.

DOI:10.1016/j.colsurfb.2011.10.048

Abstract

Spherical plant viruses like the tomato bushy stunt virus (TBSV) allow for multiple applications in nanotechnology due to their shape. In this article, different types of the virus were created by extending coat protein (CP) at carboxylic termini with 2 different charged amino acids by point mutation. The obtained CPs carried 6 aspartic acid (negative charge) and 4 histamine (positive charge) residues. The ability of TBSV to form self assembled monolayers with large ordered areas on native and chemically modified mica will be presented. The structural differences between layers formed by the wild type and by the genetically modified types will be discussed in detail.

摘要

球形植物病毒，如番茄丛矮病毒（TBSV），由于其形状，允许在纳米技术中有多种应用。在本文中，通过点突变在羧基末端将 2 种不同的带电氨基酸延伸到外壳蛋白（CP）上，从而产生不同类型的病毒。所得 CP 带有 6 个天冬氨酸（负电荷）和 4 个组氨酸（正电荷）残基。本文将介绍 TBSV 在天然和化学修饰云母上形成具有大有序面积的自组装单层的能力。将详细讨论由野生型和遗传修饰型形成的层之间的结构差异。