一种用于检测少突胶质前体细胞在星形胶质细胞上迁移的体外试验。
An in vitro assay to examine oligodendrocyte precursor cell migration on astrocytes.
作者信息
Afshari Fardad T, Fawcett James W
机构信息
Centre for Brian Repair, University of Cambridge, Cambridge, UK.
出版信息
Methods Mol Biol. 2012;814:393-9. doi: 10.1007/978-1-61779-452-0_26.
Oligodendrocyte migration is required for the myelination of axons during development and also following demyelinating lesions of the central nervous system. Oligodendrocytes arise from oligodendrocyte precursor cells (OPCs) which are present within the brain and spinal cord. To reach the demyelinating lesions, OPCs have to migrate through a dense meshwork of inhibitory astrocytes. Therefore, interactions between these two cell types are of great importance in myelination. To facilitate the study of mechanisms underlying these interactions, in vitro co-culture assays of oligodendrocyte-astrocytes have been developed. In this chapter we describe the methodology for a co-culture system known as the inverted coverslip migration assay, which has been used to study the effect of astrocytes on oligodendrocyte migratory behaviour.
在发育过程中以及中枢神经系统发生脱髓鞘损伤后，轴突的髓鞘形成需要少突胶质细胞迁移。少突胶质细胞起源于存在于脑和脊髓中的少突胶质前体细胞（OPC）。为了到达脱髓鞘损伤部位，OPC必须穿过由抑制性星形胶质细胞构成的致密网络。因此，这两种细胞类型之间的相互作用在髓鞘形成中非常重要。为了便于研究这些相互作用的潜在机制，已经开发了少突胶质细胞-星形胶质细胞的体外共培养试验。在本章中，我们描述了一种称为倒置盖玻片迁移试验的共培养系统的方法，该方法已被用于研究星形胶质细胞对少突胶质细胞迁移行为的影响。
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