Vasylyk Iu V, Lushchak V I
Ukr Biokhim Zh (1999). 2011 Jul-Sep;83(4):94-103.
The effect of sodium chloride on general morphometrical parameters of seedlings, and biochemical parameters in the leaves of corn seedlings was studied. Exposure to 100 and 200 mM NaCl slowed down the growth of stem and roots, whereas 100 and 200 mM NaCl during 24 h enhanced the concentration of chlorophylls, carotenoids, anthocyans, and thiobarbituric acid reactive substances. The decrease in protein carbonyl groups was found at 24-hour exposure to 200 mM salt. The treatment during 24, 48 and 72 h to 200 mM salt increased the level of total and high molecular mass thiols, whereas low molecular mass thiol content was by 20-25% higher at 48 h exposure to all used salt concentrations. The activity of guaiacol peroxidase was higher only at 24 h exposure to 100 and 200 mM salt, and catalase--at 50 mM during 48 h. At 72-hour exposure, catalase activity was by 27 and 41% higher in seedlings, exposed to 50 and 200 mM NaCl, respectively. Therefore, it is concluded the plant exposure to 50-200 mM salt initially developed oxidative stress, inducing adaptive response--an increase in antioxidant potential and efficiency of systems of energy production. That results in plant adaptation to unfavourable conditions.
研究了氯化钠对玉米幼苗一般形态参数及叶片生化参数的影响。暴露于100和200 mM氯化钠会减缓茎和根的生长,而在24小时内施加100和200 mM氯化钠会提高叶绿素、类胡萝卜素、花青素和硫代巴比妥酸反应性物质的浓度。在暴露于200 mM盐24小时时发现蛋白质羰基含量下降。在24、48和72小时内用200 mM盐处理会增加总硫醇和高分子量硫醇的水平,而在暴露于所有使用的盐浓度48小时时,低分子量硫醇含量高20 - 25%。愈创木酚过氧化物酶的活性仅在暴露于100和200 mM盐24小时时较高,过氧化氢酶在48小时内暴露于50 mM时活性较高。在暴露72小时时,暴露于50和200 mM氯化钠的幼苗中过氧化氢酶活性分别高27%和41%。因此,可以得出结论,植物暴露于50 - 200 mM盐时最初会产生氧化应激,诱导适应性反应——抗氧化潜力和能量产生系统效率增加。这导致植物适应不利条件。