Tsukamoto T, Sonenberg M
J Cyclic Nucleotide Res. 1979;5(2):153-9.
The relative efficiency of 1,N6-etheno-2aza-adenosine 3', 5'-monophosphate (cyclic 2-aza-epsilon AMP), 1,N6-etenoadenosine 3', 5'-monophosphate (cyclic epsilon AMP) and cyclic AMP in activation of membrane protein kinase and binding to membrane was examined using isolated membranes from human erythrocytes. Cyclic 2-aza-epsilon AMP was 81% as active as cyclic AMP in erythrocyte membrane binding and activation of membrane protein kinase. On the other hand, cyclic epsilon AMP was 37% as active toward membrane protein kinase and 29% toward membrane cyclic AMP binding. Since we have previously shown that the fluorescence of cyclic 2-aza-epsilon AMP is highly sensitive to the polarity of solvents, the high efficiency of cyclic 2-aza-epsilon AMP to substitute for cyclic amp suggests that it may be a suitable microenvironmental fluorescent probe for cyclic AMP binding sites.
利用人红细胞的分离膜，研究了1,N6-乙烯基-2-氮杂腺苷3',5'-单磷酸（环2-氮杂-ε-AMP）、1,N6-乙烯基腺苷3',5'-单磷酸（环ε-AMP）和环AMP在激活膜蛋白激酶及与膜结合方面的相对效率。环2-氮杂-ε-AMP在红细胞膜结合和激活膜蛋白激酶方面的活性为环AMP的81%。另一方面，环ε-AMP对膜蛋白激酶的活性为37%，对膜环AMP结合的活性为29%。由于我们之前已表明环2-氮杂-ε-AMP的荧光对溶剂极性高度敏感，环2-氮杂-ε-AMP替代环AMP的高效率表明它可能是用于环AMP结合位点的合适微环境荧光探针。
