诱变剂之间相互作用的分析，II. 酿酒酵母中甲基磺酸乙酯与紫外线的相互作用
Analysis of interactions between mutagens, II. Ethyl methanesulfonate and ultraviolet light in Saccharomyces cerevisiae.
作者信息
Ager D D, Haynes R H
机构信息
Department of Biology, York University, Toronto, Ont., Canada.
出版信息
Mutat Res. 1990 Oct;232(2):327-36. doi: 10.1016/0027-5107(90)90139-u.
The results of this study indicate the existence of a strong interaction between ethyl methanesulfonate (EMS) and ultraviolet light (UV) for cell killing in the yeast Saccharomyces cerevisiae. Conversely, mutation and gene conversion frequencies observed for the combined treatment of EMS and UV do not deviate significantly from that expected on the basis of simple additivity. Studies involving repair-deficient mutants (rad mutants) reveal that the synergistic interaction for cell killing depends on RAD52 function (recombinational repair), but not on RAD3 function (excision repair). On the basis of this analysis, the interaction between EMS and UV in S. cerevisiae might arise from the inhibition of double-strand break repair by one, or both agents.
本研究结果表明，在酿酒酵母中，甲磺酸乙酯（EMS）和紫外线（UV）在细胞杀伤方面存在强烈的相互作用。相反，EMS和UV联合处理所观察到的突变和基因转换频率与基于简单加性预期的频率并无显著偏差。涉及修复缺陷型突变体（rad突变体）的研究表明，细胞杀伤的协同相互作用取决于RAD52功能（重组修复），而不取决于RAD3功能（切除修复）。基于这一分析，酿酒酵母中EMS和UV之间的相互作用可能源于其中一种或两种试剂对双链断裂修复的抑制。
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