Department of Pathology, University of Kentucky, Lexington, Kentucky, USA.
J Neuropathol Exp Neurol. 2012 Jan;71(1):73-82. doi: 10.1097/NEN.0b013e318240fa65.
1p/19q codeletion is a favorable prognostic marker of oligodendrogliomas. Although fluorescence in situ hybridization (FISH) and microsatellite-based polymerase chain reaction (PCR) for loss of heterozygosity (LOH) are common methods to test for 1p/19q codeletion, it is unclear which test is better at prognostic stratification. This study analyzed outcomes of 111 oligodendrogliomas with both 1p/19q FISH and LOH done at the time of diagnosis. Overall concordance between the 2 assays was 81.1%. In grade III oligodendrogliomas, LOH was better than FISH at survival stratification (p < 0.0001 for LOH vs p = 0.02 for FISH), although increasing the stringency of FISH interpretation criteria improved concordance and prognostic power. Oligodendrogliomas that were 1p/19q-codeleted by FISH but also had 10q LOH were negative for 1p/19q codeletion by PCR analysis in more than 70% of cases, with very poor survival in the grade III subset. Thus, although PCR-based LOH is a better stratifier of 1p/19q status, FISH still has clinical and prognostic utility, especially if 10q data can be incorporated.
1p/19q 缺失是少突胶质细胞瘤的有利预后标志物。荧光原位杂交（FISH）和基于微卫星的聚合酶链反应（PCR）检测杂合性丢失（LOH）是检测 1p/19q 缺失的常用方法，但哪种方法更适合预后分层尚不清楚。本研究分析了 111 例同时进行 1p/19q FISH 和 LOH 检测的少突胶质细胞瘤的结果。两种检测方法的总体一致性为 81.1%。在 III 级少突胶质细胞瘤中，LOH 在生存分层方面优于 FISH（LOH 优于 FISH，p < 0.0001，而 FISH 优于 FISH，p = 0.02），尽管增加 FISH 解释标准的严格性可以提高一致性和预测能力。通过 FISH 检测到的 1p/19q 缺失但也存在 10q LOH 的少突胶质细胞瘤，在超过 70%的病例中通过 PCR 分析为 1p/19q 缺失阴性，III 级亚组的生存率非常差。因此，尽管基于 PCR 的 LOH 是 1p/19q 状态更好的分层因素，但 FISH 仍然具有临床和预后价值，尤其是如果可以纳入 10q 数据。
