Department of Materials Science and Engineering, University of Illinois, Urbana-Champaign, 1304 W. Green Street, Urbana, IL 61801, USA.
Angew Chem Int Ed Engl. 2012 Jan 27;51(5):1143-7. doi: 10.1002/anie.201104262. Epub 2011 Dec 7.
[Image: see text] Poly(γ-(4-vinylbenzyl)-l-glutamate) (PVBLG) served as a bioactive and reactive template for the generation of a library of cationic α-helical polypeptides for gene delivery. The top performing polymer outperformed 25-kDa polyethylenimine by 12-fold. Preliminary data indicates that helicity of these cationic polypeptides is essential for their improved performance, with enhanced membrane disruption a likely source of their transfection efficiency.
聚(γ-(4-乙烯基苄基)-l-谷氨酸酯)(PVBLG)作为生物活性和反应性模板,用于生成用于基因传递的阳离子α-螺旋多肽文库。表现最佳的聚合物比 25kDa 的聚乙烯亚胺高出 12 倍。初步数据表明,这些阳离子多肽的螺旋性对于其性能的提高是至关重要的,增强的膜破坏可能是其转染效率的来源。