全基因组 microRNA 谱分析鉴定 miR-378 作为胃癌早期检测的血清生物标志物。
Genome-wide microRNA profiles identify miR-378 as a serum biomarker for early detection of gastric cancer.
机构信息
National Engineering Center for Biochip at Shanghai, Shanghai, PR China.
出版信息
Cancer Lett. 2012 Mar 28;316(2):196-203. doi: 10.1016/j.canlet.2011.10.034. Epub 2011 Nov 2.
Recent studies demonstrated that in several human malignancies aberrant expression profiles of circulating microRNAs (miRNAs) anticipate great cancer diagnostic potential. Here we showed that serum miR-378 could serve as a novel noninvasive biomarker in gastric cancer (GC) detection. Genome-wide miRNA expression profiles followed with Real-Time quantitative RT-PCR (qRT-PCR) assays revealed that miR-187(*), miR-371-5p and miR-378 were significantly elevated in GC patients. Further validation indicated that miR-378 alone could yields a ROC curve area of 0.861 with 87.5% sensitivity and 70.73% specificity in discriminating GC patients from healthy controls. Collectively, these data support our contention that serum miR-378 has strong potential as a novel noninvasive biomarker in gastric cancer detection.
最近的研究表明，在几种人类恶性肿瘤中，循环 microRNAs（miRNAs）的异常表达谱预示着巨大的癌症诊断潜力。在这里，我们表明血清 miR-378 可以作为胃癌（GC）检测的新型无创生物标志物。全基因组 miRNA 表达谱结合实时定量 RT-PCR（qRT-PCR）检测显示，GC 患者中 miR-187(*)、miR-371-5p 和 miR-378 显著升高。进一步验证表明，miR-378 单独使用时，在区分 GC 患者和健康对照者方面，ROC 曲线下面积为 0.861，灵敏度为 87.5%，特异性为 70.73%。综上所述，这些数据支持我们的观点，即血清 miR-378 具有作为胃癌检测新型无创生物标志物的强大潜力。
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