Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
Cytometry A. 2012 Feb;81(2):165-8. doi: 10.1002/cyto.a.22005. Epub 2011 Dec 13.
Erythroid biology research involving rhesus macaques has been applied to several topics including malaria, hemoglobinopathy and gene therapy research. However, analyses of the rhesus red blood cells are limited by the inability to identify and sort those cells in research blood samples using flow cytometry. Here it is reported that the BRIC 6 hybridoma clone raised to the human erythroid surface molecule (referred to as CD233, Band 3, AE1, or SLC4A1) produces cross-reactive and erythroid-specific antibodies for flow cytometric detection and sorting of rhesus macaque erythrocytes.
恒河猴的红细胞生物学研究已应用于多个领域,包括疟疾、血红蛋白病和基因治疗研究。然而,由于无法使用流式细胞术在研究血液样本中鉴定和分选这些细胞,对恒河猴红细胞的分析受到限制。本文报道称,针对人红细胞表面分子(称为 CD233、Band3、AE1 或 SLC4A1)产生的 BRIC 6 杂交瘤克隆产生的抗体可与恒河猴红细胞发生交叉反应,具有红细胞特异性,可用于流式细胞术检测和分选恒河猴红细胞。