在直接同种异体识别途径中，可诱导共刺激分子/ B7h共刺激阻断导致的T细胞增殖抑制。
Inhibition of T-cell expansion caused by inducible costimulator/B7h costimulation blockade in direct allorecognition pathway.
作者信息
Du J F, Li Q-Y, Ji X Q, Chen G, Bai X, Zuo F-Y, Yu B
机构信息
Department of General Surgery, General Hospital of Beijing Military Command, Beijing, China.
出版信息
Transplant Proc. 2011 Dec;43(10):3960-3. doi: 10.1016/j.transproceed.2011.09.044.
OBJECTIVE
Inducible costimulator (ICOS)/B7h costimulation plays a crucial role in acute and chronic allograft rejection. To test the role of the ICOS signal in T-cell activation and expansion, we used ICOS-Fc-targeted B cells as donor antigen presenting cells to challenge the allogeneic response in vitro.
METHODS
In vitro, the binding of ICOS-Fc with B7h on splenic B cells was confirmed by flow cytometry analysis. To evaluate the capacity of ICOS-Fc-targeted B cells to elicit an allogeneic response in vitro, we performed mixed lymphocyte reactions.
RESULTS
The binding of B7h on splenic B cells by ICOS-Fc was confirmed at a saturating concentration of 100 μg/mL. Blockade of ICOS/B7h in direct allorecognition depressed proliferation of alloreactive T cells in vitro.
CONCLUSIONS
ICOS/B7h signal plays an important role in direct allorecognition, eliciting allogeneic responses in vitro.
目的
诱导性共刺激分子（ICOS）/B7h共刺激在急性和慢性同种异体移植排斥反应中起关键作用。为了测试ICOS信号在T细胞活化和扩增中的作用，我们使用ICOS-Fc靶向的B细胞作为供体抗原呈递细胞在体外激发同种异体反应。
方法
在体外，通过流式细胞术分析证实ICOS-Fc与脾B细胞上的B7h结合。为了评估ICOS-Fc靶向的B细胞在体外引发同种异体反应的能力，我们进行了混合淋巴细胞反应。
结果
在100μg/mL的饱和浓度下证实了ICOS-Fc与脾B细胞上B7h的结合。在直接同种异体识别中阻断ICOS/B7h可抑制体外同种反应性T细胞的增殖。
结论
ICOS/B7h信号在直接同种异体识别中起重要作用，在体外引发同种异体反应。
Zotero 插件