基于液晶的乙型肝炎抗体检测免疫分析。

Liquid crystal-based immunoassays for detecting hepatitis B antibody.

机构信息

Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Singapore.

出版信息

Anal Biochem. 2012 Feb 1;421(1):321-3. doi: 10.1016/j.ab.2011.11.007. Epub 2011 Nov 16.

DOI:10.1016/j.ab.2011.11.007

Abstract

Conventional immunoassays are often fluorescence- or enzyme-based and require additional readout systems. Here, we report a liquid crystal (LC)-based immunoassay that features a new signal transduction mechanism, whereby the test results appear as colorful spots and can be viewed with the naked eye directly. By using the different colors of LC spots, we can estimate the range of anti-hepatitis B surface antigen concentrations between 300 and 15 nM. Because additional labeling process and readout systems are not required in this immunoassay, it is more suitable for point-of-care diagnosis of hepatitis B in resource-limited regions.

摘要

传统的免疫分析方法通常基于荧光或酶，并需要额外的读出系统。在这里，我们报告了一种基于液晶（LC）的免疫分析方法，其具有新的信号转导机制，测试结果以彩色斑点的形式出现，可以直接用肉眼观察。通过使用 LC 斑点的不同颜色，我们可以估计抗乙型肝炎表面抗原浓度的范围在 300 到 15 nM 之间。由于这种免疫分析方法不需要额外的标记过程和读出系统，因此更适合在资源有限的地区进行乙型肝炎的即时诊断。