褐球固氮菌对芳香物质的利用

Utilization of aromatic substances by Azotobacter chroococcum.

作者信息

Balajee S, Mahadevan A

机构信息

Centre for Advanced Study in Botany, University of Madras, India.

出版信息

Res Microbiol. 1990 Jun;141(5):577-84. doi: 10.1016/0923-2508(90)90022-i.

DOI:10.1016/0923-2508(90)90022-i

PMID:2218062

Abstract

Azotobacter chroococcum utilized catechol, protocatechuic acid and gentisic acid as sole carbon source. Rothera's test indicated the ortho cleavage of phenols. The cells displayed high levels of dioxygenase activity. Catechol was cleaved by the ortho pathway mediated by catechol 1,2-dioxygenase. Protocatechuate and gentisic acid were also cleaved by the ortho pathway and cells grown in protocatechuate and gentisic acid displayed protocatechuate, 3,4-dioxygenase and gentisate dioxygenase activity, respectively.

摘要

褐球固氮菌利用儿茶酚、原儿茶酸和龙胆酸作为唯一碳源。罗瑟拉试验表明酚类发生邻位裂解。这些细胞表现出高水平的双加氧酶活性。儿茶酚通过由儿茶酚1,2-双加氧酶介导的邻位途径裂解。原儿茶酸和龙胆酸也通过邻位途径裂解，在原儿茶酸和龙胆酸中生长的细胞分别表现出原儿茶酸3,4-双加氧酶和龙胆酸双加氧酶活性。