Hayes B E, Clarke D E
Division of Pharmaceutical Sciences, College of Pharmacy and Health Sciences, Texas Southern University, Houston 77004.
Res Commun Chem Pathol Pharmacol. 1990 Jul;69(1):71-83.
Semicarbazide-sensitive amine oxidase (SSAO) activity was investigated in serum and tissues of streptozotocin diabetic rats. Diabetes was induced by a single intraperitoneal injection of streptozotocin (STZ, 60 mg/kg) and rats were killed at 1, 3, 7, 9, 14, 28 and 56 days after treatment. STZ increased serum glucose and serum SSAO activity at all time points with a maximal increase (2 to 3 fold) at day 7. Kidney SSAO activity showed significant increases on days 9 and 14 (2.5 and 4 fold, respectively). SSAO activity of aorta, lung and pancreas was not changed significantly. Kinetic analysis showed that the elevation in both serum and kidney SSAO activity was due to an increased Vmax with no change in Km. SSAO activity appears to be selectively increased in the serum and kidney of STZ diabetic rats which may be indicative of a relationship between serum SSAO and early renovascular damage in this animal model of diabetes mellitus.
研究了链脲佐菌素诱导的糖尿病大鼠血清和组织中的氨基脲敏感胺氧化酶（SSAO）活性。通过单次腹腔注射链脲佐菌素（STZ，60 mg/kg）诱导糖尿病，在治疗后1、3、7、9、14、28和56天处死大鼠。在所有时间点，STZ均增加血清葡萄糖和血清SSAO活性，在第7天增加最大（2至3倍）。肾脏SSAO活性在第9天和第14天显著增加（分别为2.5倍和4倍）。主动脉、肺和胰腺的SSAO活性无显著变化。动力学分析表明，血清和肾脏SSAO活性的升高是由于Vmax增加而Km不变。在链脲佐菌素诱导的糖尿病大鼠的血清和肾脏中，SSAO活性似乎选择性增加，这可能表明在该糖尿病动物模型中血清SSAO与早期肾血管损伤之间存在关联。
