Pretorius E, Oberholzer H M, van der Spuy W J, Franz R C
Department of Physiology, School of Medicine, Faculty of Health Sciences, University of Pretoria, South Africa.
Hematology. 2011 Nov;16(6):337-40. doi: 10.1179/102453311X13127324303515.
Fibrin plays a vital role in the coagulation process and fibrin fiber morphology can be studied using ultrastructural techniques. When studying the ultrastructure of fibrin networks, thrombin may be added to the plasma, ensuing fibrin network formation. The question that arises is whether there are differences in morphology when thrombin is added to plasma, versus morphology observed when plasma from citrated or recalcified citrated whole blood, is studied. The current study therefore aimed to compare ultrastructure of platelets and fibrin networks from these three techniques. Results indicated comparable platelet ultrastructure between smears formed from the plasma of citrated blood and that of the citrated recalcified blood. This method might give us further information regarding the 'natural state' fibrin assembly and association with platelets, when studying haemostasis. However, when studying the ultrastructure of fibrin networks, the addition of thrombin is necessary to form an expansive, fully coagulated layer of fibrin fibers.
纤维蛋白在凝血过程中起着至关重要的作用，并且可以使用超微结构技术研究纤维蛋白纤维的形态。在研究纤维蛋白网络的超微结构时，可以向血浆中添加凝血酶，从而形成纤维蛋白网络。由此产生的问题是，将凝血酶添加到血浆中时的形态与研究枸橼酸盐化或重新钙化的枸橼酸盐化全血的血浆时观察到的形态是否存在差异。因此，本研究旨在比较这三种技术所得到的血小板和纤维蛋白网络的超微结构。结果表明，枸橼酸盐化血液的血浆和枸橼酸盐化再钙化血液的血浆所制成的涂片之间，血小板超微结构具有可比性。在研究止血过程时，这种方法可能会为我们提供有关“自然状态”下纤维蛋白组装以及与血小板关联的更多信息。然而，在研究纤维蛋白网络的超微结构时，添加凝血酶对于形成一个广泛的、完全凝固的纤维蛋白纤维层是必要的。
