Adams Elizabeth, Emerson Danielle, Croker Sean, Kim Hye-Seon, Modla Shannon, Kang Seogchan, Czymmek Kirk
Delaware Biotechnology Institute, University of Delaware, Newark, DE, USA.
Methods Mol Biol. 2012;835:151-64. doi: 10.1007/978-1-61779-501-5_10.
One of the primary roles of the cell surface is to provide an effective barrier to various external environmental factors. Specifically, the surface properties of organisms serve as a critical obstacle to pathogen attack. Since its inception, Atomic Force Microscopy (AFM) has enabled nanoscale imaging of cell surfaces in their native state. However AFM has yet to be systematically applied toward resolving surface features and the forces underpinning plant-fungal interactions. In an effort to understand the physical forces involved at the plant-microbe interface, we describe a method for the attachment of fungal spores to AFM tips and the subsequent measurement of unbinding forces between spores with a range of substrates and plant surfaces under physiologically relevant conditions. Investigations of binding events using AFM offer an unexplored, sensitive, and quantitative method for analyzing host-pathogen/microbe-surface interactions.
细胞表面的主要作用之一是为各种外部环境因素提供有效的屏障。具体而言，生物体的表面特性是病原体攻击的关键障碍。自问世以来，原子力显微镜（AFM）已能够对天然状态下的细胞表面进行纳米级成像。然而，AFM尚未被系统地应用于解析植物-真菌相互作用的表面特征和潜在作用力。为了了解植物-微生物界面涉及的物理力，我们描述了一种将真菌孢子附着到AFM尖端的方法，并随后在生理相关条件下测量孢子与一系列底物和植物表面之间的解离力。使用AFM对结合事件进行研究，为分析宿主-病原体/微生物-表面相互作用提供了一种未被探索的、灵敏且定量的方法。
