真菌效应蛋白在毕赤酵母中的异源表达。

Heterologous production of fungal effectors in Pichia pastoris.

作者信息

Kombrink Anja

机构信息

Laboratory of Phytopathology, Wageningen University, Wageningen, The Netherlands.

出版信息

Methods Mol Biol. 2012;835:209-17. doi: 10.1007/978-1-61779-501-5_13.

DOI:10.1007/978-1-61779-501-5_13

PMID:22183656

Abstract

In this chapter a method for the heterologous production of fungal proteins in the yeast Pichia pastoris is described. Starting with cloning of the sequence encoding the gene of interest into the expression vector, this protocol describes P. pastoris transformation, production of the protein in a fermentor, and purification of the protein. This method has successfully been used for the production of a number of fungal effector proteins.

摘要

本章介绍了一种在巴斯德毕赤酵母中异源生产真菌蛋白的方法。从将编码目标基因的序列克隆到表达载体开始，本方案描述了巴斯德毕赤酵母的转化、在发酵罐中生产蛋白以及蛋白的纯化。该方法已成功用于生产多种真菌效应蛋白。