Jagiellonian University, Medical College, Pharmaceutical Faculty, Department of Inorganic and Analytical Chemistry, Medyczna 9, 30-688, Krakow, Poland.
Photochem Photobiol Sci. 2012 Feb;11(2):351-7. doi: 10.1039/c1pp05259d. Epub 2011 Dec 21.
Photostability of moxifloxacin (MOXI) after UVA irradiation in solutions and solid phase, with and without participation of Cu(II), Zn(II), Al(III), and Fe(III) was tested. The studies were carried out by the TLC-densitometric method and LC-MS/MS method. Elaborated and validated chromatography-densitometric method was used for assaying. It was shown that the number and type of photoproducts depend on the environment and type of the metal ion. The studied ions enhanced the degradation of MOXI in solutions, and the influence of Cu(II) and Fe(III) ions was higher than that of Zn(II) and Al(III) ions. In solid phase, in contrast to solutions, all metal ions decreased the photodegradation, however the influence of ions, Al(III) and Zn(II), was weaker than that of Cu(II) and Fe(III) ions. Identification of the degradation products performed with LC-MS/MS and (1)H NMR identified them as: 1-cyclopropyl-6-fluoro-7-amino-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid, 1-cyclopropyl-6-fluoro-8-methoxy-4-oxo-7-(2-oxo-octahydro-6H-pyrrolo[3,4-b]pyridine-6-yl)-1,4-dihydroquinoline-3-carboxylic acid, 7-[3-hydroxyamino-4-(2-carboxyethyl)pyrrolidin-1-yl]-1-cyclopropyl-6-fluoro-8-methoxy-4-oxo-1,4-dihydroquinoline-3-carboxylic acid.
在有和没有 Cu(II)、Zn(II)、Al(III)和 Fe(III)参与的情况下，测试了莫西沙星（MOXI）在溶液和固相中的 UVA 照射后的光稳定性。研究采用 TLC-密度测定法和 LC-MS/MS 法进行。使用详细验证的色谱-密度测定法进行测定。结果表明，光产物的数量和类型取决于环境和金属离子的类型。研究离子增强了 MOXI 在溶液中的降解，Cu(II)和 Fe(III)离子的影响高于 Zn(II)和 Al(III)离子。在固相中，与溶液相反，所有金属离子都降低了光降解，但是离子、Al(III)和 Zn(II)的影响弱于 Cu(II)和 Fe(III)离子。用 LC-MS/MS 和 (1)H NMR 对降解产物进行鉴定，确定它们为：1-环丙基-6-氟-7-氨基-8-甲氧基-4-氧代-1,4-二氢喹啉-3-羧酸、1-环丙基-6-氟-8-甲氧基-4-氧代-7-(2-氧代-六氢吡咯并[3,4-b]吡啶-6-基)-1,4-二氢喹啉-3-羧酸、7-[3-氨基-4-(2-羧乙基)吡咯烷-1-基]-1-环丙基-6-氟-8-甲氧基-4-氧代-1,4-二氢喹啉-3-羧酸。
