酵母β-(1-3),(1-6)-葡聚糖诱导树突状细胞 1 阳性的人 B 淋巴瘤细胞系上调 CD86 的表达。
Yeast-derived beta-(1-3),(1-6)-D-glucan induces up-regulation of CD86 on dectin-1-positive human B-lymphoma cell lines.
机构信息
Medical Clinic of Oncology and Hematology, Charité University Medicine Berlin, Campus Mitte, 10117 Berlin, Germany.
出版信息
Anticancer Res. 2011 Dec;31(12):4195-9.
BACKGROUND
The knowledge of direct effects of β-glucans on tumor cells is limited. This study evaluated the impact of a soluble yeast-derived beta-(1-3),(1-6)-d-glucan, containing a fraction of aggregated sugar polymers, on viability, proliferation and expression of CD86 of the human B-cell lymphoma cell lines Daudi and Raji.
MATERIALS AND METHODS
Proliferation of carboxyfluorescein diacetate succinimidyl ester (CFSE)-stained cell lines was determined by measuring depletion of the dye and cell death was quantified by staining with propidium iodide, both by flow cytometry. Surface expression of CD86 and the beta-glucan receptors dectin-1 and complement receptor 3 (CR3) was assessed by flow cytometry.
RESULTS
Exposure to the carbohydrate increased the expression of CD86 on both dectin-1(+)CR3(-) cell lines, whereas proliferation and viability of the cells were not affected.
CONCLUSION
Yeast-derived beta-glucan lacks cytotoxic effects towards B-lymphoma cells but up-regulation of CD86 suggests maturation of the cells via dectin-1 by the carbohydrate.
背景
人们对β-葡聚糖对肿瘤细胞的直接作用知之甚少。本研究评估了一种可溶性酵母衍生的β-（1-3），（1-6）-d-葡聚糖（其中含有部分聚集糖聚合物）对人 B 细胞淋巴瘤细胞系 Daudi 和 Raji 的活力、增殖和 CD86 表达的影响。
材料和方法
通过测量染料的耗尽来通过流式细胞术测定经羧基荧光素二乙酸琥珀酰亚胺酯（CFSE）染色的细胞系的增殖，并用碘化丙啶染色定量细胞死亡。通过流式细胞术评估 CD86 和 β-葡聚糖受体 dectin-1 和补体受体 3（CR3）的表面表达。
结果
暴露于碳水化合物会增加两种 dectin-1（+）CR3（-）细胞系上 CD86 的表达，而细胞的增殖和活力不受影响。
结论
酵母衍生的β-葡聚糖对 B 淋巴瘤细胞没有细胞毒性作用，但 CD86 的上调表明细胞通过碳水化合物被 dectin-1 成熟。
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