[Purification and properties of two forms of soluble 3',5'-AMP-dependent protein kinase from rabbit myocardium].

Two forms of 3' : 5'-AMP-dependent protein kinase were purified from the rabbit myocardium. The concentrations of 3' : 5'-AMP-necessary for maximum stimulation of form I and II were the same but the second form of the enzyme was activated by this concentration to a greater extent. The seeming Km by ATP for the both forms in the presence of 3' : 5'-AMP is found to be 8.3.10(-6)M. For enzyme form I the seeming Km by histone H1 was 0.43 mg/ml and for form II--0.67 mg/ml.